Sergei Zolotukhin Department of Pediatrics University of Florida Biography Publications Institution JoVE Articles Sergei Zolotukhin has not added a biography. If you are Sergei Zolotukhin and would like to personalize this page please email our Author Liaison for assistance. Publications Rational Design of AAV-rh74, AAV3B, and AAV8 with Limited Liver Targeting Viruses. Oct, 2023 | Pubmed ID: 38005848 Exploring the Comprehensive Kozak Sequence Landscape for AAV Production in Sf9 System Viruses. Sep, 2023 | Pubmed ID: 37896760 Intravitreal Injection of a Rationally Designed AAV Capsid Library in Non-human Primate Identifies Variants with Enhanced Retinal Transduction and Neutralizing Antibody Evasion Molecular Therapy : the Journal of the American Society of Gene Therapy. Dec, 2023 | Pubmed ID: 37814449 Characterization of a Bioengineered AAV3B Capsid Variant with Enhanced Hepatocyte Tropism and Immune Evasion Human Gene Therapy. Apr, 2023 | Pubmed ID: 36950804 Identification of Novel Retinal Pericyte-Targeting RAAV Vectors Through Directed Evolution Translational Vision Science & Technology. Aug, 2022 | Pubmed ID: 36018583 AAV Capsid Design: A Goldilocks Challenge Trends in Molecular Medicine. Mar, 2022 | Pubmed ID: 35093287 Scratching the Surface of RGD-directed AAV Capsid Engineering Molecular Therapy : the Journal of the American Society of Gene Therapy. Nov, 2021 | Pubmed ID: 34699781 Y1 Receptors Modulate Taste-related Behavioral Responsiveness in Male Mice to Prototypical Gustatory Stimuli Hormones and Behavior. Nov, 2021 | Pubmed ID: 34509673 A Comprehensive Study of a 29-capsid AAV Library in a Non-human Primate Central Nervous System Molecular Therapy : the Journal of the American Society of Gene Therapy. Sep, 2021 | Pubmed ID: 34298128 Mixture Interactions at Mammalian Olfactory Receptors Are Dependent on the Cellular Environment Scientific Reports. Apr, 2021 | Pubmed ID: 33927269 The CRTC1-MAML2 Fusion is the Major Oncogenic Driver in Mucoepidermoid Carcinoma JCI Insight. Apr, 2021 | Pubmed ID: 33830080 Applying Machine Learning to Predict Viral Assembly for Adeno-associated Virus Capsid Libraries Molecular Therapy. Methods & Clinical Development. Mar, 2021 | Pubmed ID: 33511242 Engineering and Selection of a Novel AAV3B Variant with High Hepatocyte Tropism and Reduced Seroreactivity Molecular Therapy. Methods & Clinical Development. Dec, 2020 | Pubmed ID: 33145371 Removal of Endotoxin from RAAV Samples Using a Simple Detergent-Based Protocol Molecular Therapy. Methods & Clinical Development. Dec, 2019 | Pubmed ID: 31649960 Taste Receptor Cells in Mice Express Receptors for the Hormone Adiponectin Chemical Senses. Jul, 2019 | Pubmed ID: 31125082 Achieving High-Yield Production of Functional AAV5 Gene Delivery Vectors Via Fedbatch in an Insect Cell-One Baculovirus System Molecular Therapy. Methods & Clinical Development. Jun, 2019 | Pubmed ID: 30886878 Helper-free Production of Laboratory Grade AAV and Purification by Iodixanol Density Gradient Centrifugation Molecular Therapy. Methods & Clinical Development. Sep, 2018 | Pubmed ID: 30073177 Direct Head-to-Head Evaluation of Recombinant Adeno-associated Viral Vectors Manufactured in Human Versus Insect Cells Molecular Therapy : the Journal of the American Society of Gene Therapy. Dec, 2017 | Pubmed ID: 28890324 An Immune-Competent Murine Model to Study Elimination of AAV-Transduced Hepatocytes by Capsid-Specific CD8 T Cells Molecular Therapy. Methods & Clinical Development. Jun, 2017 | Pubmed ID: 28480313 Evaluation of Engineered AAV Capsids for Hepatic Factor IX Gene Transfer in Murine and Canine Models Journal of Translational Medicine. May, 2017 | Pubmed ID: 28460646 OneBac 2.0: Sf9 Cell Lines for Production of AAV1, AAV2, and AAV8 Vectors with Minimal Encapsidation of Foreign DNA Human Gene Therapy Methods. Feb, 2017 | Pubmed ID: 28125901 Limited ATF4 Expression in Degenerating Retinas with Ongoing ER Stress Promotes Photoreceptor Survival in a Mouse Model of Autosomal Dominant Retinitis Pigmentosa PloS One. 2016 | Pubmed ID: 27144303 Prader-Willi Critical Region, a Non-Translated, Imprinted Central Regulator of Bone Mass: Possible Role in Skeletal Abnormalities in Prader-Willi Syndrome PloS One. 2016 | Pubmed ID: 26824232 Snord116 is Critical in the Regulation of Food Intake and Body Weight Scientific Reports. Jan, 2016 | Pubmed ID: 26726071 Altering Tropism of RAAV by Directed Evolution Methods in Molecular Biology (Clifton, N.J.). 2016 | Pubmed ID: 26611585 High-accuracy Biodistribution Analysis of Adeno-associated Virus Variants by Double Barcode Sequencing Molecular Therapy. Methods & Clinical Development. 2015 | Pubmed ID: 26793739 Adeno-Associated Virus Type 2 and Hepatocellular Carcinoma? Human Gene Therapy. Dec, 2015 | Pubmed ID: 26690810 E Pluribus Unum: 50 Years of Research, Millions of Viruses, and One Goal--Tailored Acceleration of AAV Evolution Molecular Therapy : the Journal of the American Society of Gene Therapy. Dec, 2015 | Pubmed ID: 26388463 OneBac 2.0: Sf9 Cell Lines for Production of AAV5 Vectors with Enhanced Infectivity and Minimal Encapsidation of Foreign DNA Human Gene Therapy. Oct, 2015 | Pubmed ID: 26134901 A Simplified Purification Protocol for Recombinant Adeno-associated Virus Vectors Molecular Therapy. Methods & Clinical Development. 2014 | Pubmed ID: 26015974 Identification of Low-expressing Transcripts of the NPY Receptors' Family in the Murine Lingual Epithelia Methods in Molecular Biology (Clifton, N.J.). 2014 | Pubmed ID: 25218388 Vector Design Tour De Force: Integrating Combinatorial and Rational Approaches to Derive Novel Adeno-associated Virus Variants Molecular Therapy : the Journal of the American Society of Gene Therapy. Nov, 2014 | Pubmed ID: 25048217 OneBac: Platform for Scalable and High-titer Production of Adeno-associated Virus Serotype 1-12 Vectors for Gene Therapy Human Gene Therapy. Mar, 2014 | Pubmed ID: 24299301 Salivary Peptide Tyrosine-tyrosine 3-36 Modulates Ingestive Behavior Without Inducing Taste Aversion The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Nov, 2013 | Pubmed ID: 24259562 Modulation of Taste Responsiveness by the Satiation Hormone Peptide YY FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. Dec, 2013 | Pubmed ID: 24043261 Structural Insights into Adeno-associated Virus Serotype 5 Journal of Virology. Oct, 2013 | Pubmed ID: 23926356 Development of Novel Recombinant AAV Vectors and Strategies for the Potential Gene Therapy of Hemophilia Journal of Genetic Syndromes & Gene Therapy. Jan, 2012 | Pubmed ID: 23264889 Distribution of Y-receptors in Murine Lingual Epithelia PloS One. 2012 | Pubmed ID: 23050020 High-efficiency Transduction of Human Monocyte-derived Dendritic Cells by Capsid-modified Recombinant AAV2 Vectors Vaccine. Jun, 2012 | Pubmed ID: 22497875 Structural Insight into the Unique Properties of Adeno-associated Virus Serotype 9 Journal of Virology. Jun, 2012 | Pubmed ID: 22496238 Neutralizing Antibodies Against AAV Serotypes 1, 2, 6, and 9 in Sera of Commonly Used Animal Models Molecular Therapy : the Journal of the American Society of Gene Therapy. Jan, 2012 | Pubmed ID: 21915102 Salivary PYY: a Putative Bypass to Satiety PloS One. 2011 | Pubmed ID: 22028819 High-efficiency Transduction and Correction of Murine Hemophilia B Using AAV2 Vectors Devoid of Multiple Surface-exposed Tyrosines Molecular Therapy : the Journal of the American Society of Gene Therapy. Dec, 2010 | Pubmed ID: 20736929 An Animal Model of PDH Deficiency Using AAV8-siRNA Vector-mediated Knockdown of Pyruvate Dehydrogenase E1α Molecular Genetics and Metabolism. Oct-Nov, 2010 | Pubmed ID: 20685142 Production, Purification and Preliminary X-ray Crystallographic Studies of Adeno-associated Virus Serotype 9 Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Jul, 2009 | Pubmed ID: 19574648 An Inducible System for Highly Efficient Production of Recombinant Adeno-associated Virus (rAAV) Vectors in Insect Sf9 Cells Proceedings of the National Academy of Sciences of the United States of America. Mar, 2009 | Pubmed ID: 19279219 High-efficiency Transduction of the Mouse Retina by Tyrosine-mutant AAV Serotype Vectors Molecular Therapy : the Journal of the American Society of Gene Therapy. Mar, 2009 | Pubmed ID: 19066593 Tyrosine-phosphorylation of AAV2 Vectors and Its Consequences on Viral Intracellular Trafficking and Transgene Expression Virology. Nov, 2008 | Pubmed ID: 18834608 Next Generation of Adeno-associated Virus 2 Vectors: Point Mutations in Tyrosines Lead to High-efficiency Transduction at Lower Doses Proceedings of the National Academy of Sciences of the United States of America. Jun, 2008 | Pubmed ID: 18511559 Optimization of Recombinant Adeno-associated Viral Vectors for Human Beta-globin Gene Transfer and Transgene Expression Human Gene Therapy. Apr, 2008 | Pubmed ID: 18399730 Synergy Between Leptin Therapy and a Seemingly Negligible Amount of Voluntary Wheel Running Prevents Progression of Dietary Obesity in Leptin-resistant Rats Diabetes. Mar, 2008 | Pubmed ID: 18086903 Production, Purification and Preliminary X-ray Crystallographic Studies of Adeno-associated Virus Serotype 7 Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Dec, 2007 | Pubmed ID: 18084098 Structure of Adeno-associated Virus Serotype 8, a Gene Therapy Vector Journal of Virology. Nov, 2007 | Pubmed ID: 17728238 Ectopic Expression of Wnt10b Decreases Adiposity and Improves Glucose Homeostasis in Obese Rats American Journal of Physiology. Endocrinology and Metabolism. Sep, 2007 | Pubmed ID: 17578883 A Dual Role of EGFR Protein Tyrosine Kinase Signaling in Ubiquitination of AAV2 Capsids and Viral Second-strand DNA Synthesis Molecular Therapy : the Journal of the American Society of Gene Therapy. Jul, 2007 | Pubmed ID: 17440440 Production, Purification and Preliminary X-ray Crystallographic Studies of Adeno-associated Virus Serotype 1 Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Dec, 2006 | Pubmed ID: 17142915 Production, Purification, Crystallization and Preliminary X-ray Structural Studies of Adeno-associated Virus Serotype 5 Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Oct, 2005 | Pubmed ID: 16511195 Production, Purification, Crystallization and Preliminary X-ray Analysis of Adeno-associated Virus Serotype 8 Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Jun, 2005 | Pubmed ID: 16511095 Successful Production of Pseudotyped RAAV Vectors Using a Modified Baculovirus Expression System Molecular Therapy : the Journal of the American Society of Gene Therapy. Dec, 2005 | Pubmed ID: 16213797 Gene Therapy for Obesity Expert Opinion on Biological Therapy. Mar, 2005 | Pubmed ID: 15833072 Efficient Transduction of Vascular Endothelial Cells with Recombinant Adeno-associated Virus Serotype 1 and 5 Vectors Human Gene Therapy. Feb, 2005 | Pubmed ID: 15761263 Recombinant AAV Viral Vectors Pseudotyped with Viral Capsids from Serotypes 1, 2, and 5 Display Differential Efficiency and Cell Tropism After Delivery to Different Regions of the Central Nervous System Molecular Therapy : the Journal of the American Society of Gene Therapy. Aug, 2004 | Pubmed ID: 15294177 Adeno-associated Virus Type 2 VP2 Capsid Protein is Nonessential and Can Tolerate Large Peptide Insertions at Its N Terminus Journal of Virology. Jun, 2004 | Pubmed ID: 15163751 Sustained Peripheral Expression of Transgene Adiponectin Offsets the Development of Diet-induced Obesity in Rats Proceedings of the National Academy of Sciences of the United States of America. Nov, 2003 | Pubmed ID: 14617771 Identification of Amino Acid Residues in the Capsid Proteins of Adeno-associated Virus Type 2 That Contribute to Heparan Sulfate Proteoglycan Binding Journal of Virology. Jun, 2003 | Pubmed ID: 12768018 Production and Purification of Serotype 1, 2, and 5 Recombinant Adeno-associated Viral Vectors Methods (San Diego, Calif.). Oct, 2002 | Pubmed ID: 12413414 Streamlined Large-scale Production of Recombinant Adeno-associated Virus (rAAV) Vectors Methods in Enzymology. 2002 | Pubmed ID: 11883083 Transduction of Human and Mouse Pancreatic Islet Cells Using a Bicistronic Recombinant Adeno-associated Viral Vector Molecular Therapy : the Journal of the American Society of Gene Therapy. Feb, 2002 | Pubmed ID: 11829522 Efficient Photoreceptor-targeted Gene Expression in Vivo by Recombinant Adeno-associated Virus Proceedings of the National Academy of Sciences of the United States of America. Jun, 1997 | Pubmed ID: 9192666 Author Spotlight: Efficient Adeno-Associated Virus Isolation for Pre-Clinical Applications Kathryn K. Harris1, Oleksandr Kondratov1, Sergei Zolotukhin1 1Department of Pediatrics, University of Florida JoVE 66460 Medicine
Author Spotlight: Efficient Adeno-Associated Virus Isolation for Pre-Clinical Applications Kathryn K. Harris1, Oleksandr Kondratov1, Sergei Zolotukhin1 1Department of Pediatrics, University of Florida JoVE 66460 Medicine