Heilongjiang University of Chinese Medicine 2 articles published in JoVE Behavior Acupuncture Treatment in a Mouse Model of Chronic Hypoxia-Induced Cognitive Dysfunction Fang Wan*1, Zhifang Guo*2,3, Mingye Wang4, Yunlong Hou5,6, Lu Wang3,5, Wenyan Li4,7, Ning Kang2,3, Pengyu Zhu8, Mengnan Li5,6 1Heilongjiang University of Chinese Medicine, 2Hebei Provincial Key Laboratory of Luobing, 3Key Laboratory of State Administration of TCM (Cardio-Cerebral Vessel Collateral Disease), 4Hebei University of Chinese Medicine, 5Shijiazhuang Compound Traditional Chinese Medicine Technology Innovation Center, 6National Key Laboratory of Luobing Research and Innovative Chinese Medicine, 7Shijiazhuang Yiling Pharmaceutical Co., Ltd, 8The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine Here, we describe a protocol for implementing mild anesthesia and acupuncture treatment on a chronic hypoxia mouse model and conducting behavioral tests to assess the cognitive alterations post-treatment. Biochemistry Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification Anthony A. High1, Haiyan Tan1, Vishwajeeth R. Pagala1, Mingming Niu2,3, Ji-Hoon Cho1, Xusheng Wang1, Bing Bai2, Junmin Peng1,2 1 We present a protocol to accurately quantitate proteins with isobaric labelling, extensive fractionation, bioinformatics tools, and quality control steps in combination with liquid chromatography interfaced to a high-resolution mass spectrometer.