The First Affiliated Hospital of Xian Jiaotong University 1 article published in JoVE Medicine Robot-assisted Total Mesorectal Excision and Lateral Pelvic Lymph Node Dissection for Locally Advanced Middle-low Rectal Cancer Chenhao Hu*1,2,3, Zhe Zhang*1,2,3, Lei Zhang1,2,3, Ruihan Liu2,3, Jun Yan1,2,3, Qi Sun1,2,3, Guanghui Wang1, Junjun She1,2,3 1Department of General Surgery, The First Affiliated Hospital of Xian Jiaotong University, 2Center for Gut Microbiome Research, Med-X Institute, The First Affiliated Hospital of Xian Jiao tong University, 3Department of High Talent, The First Affiliated Hospital of Xian Jiaotong University The robotic technique described herein aims to detail a stepwise approach to robot-assisted total mesorectal excision and lateral pelvic lymph node dissection for locally advanced (T3/T4) rectal cancer located below the peritoneal reflection.