Hunan University of Chinese Medicine 1 article published in JoVE Medicine Mimicking Ding's Roll Method on Notexin-Induced Muscle Injury in Rats Bo Huang1, Lei Ruan1, Lanlan Wang1, Huitian Xue1, Menglong Sun1, Miaomiao Duan1, Liang Peng1 1College of Acupuncture and Tuina and Rehabilitation, Hunan University of Chinese Medicine This protocol describes a simple device that mimics Ding's roll method, establishes a rat model of skeletal muscle injury, and uses hematoxylin-eosin staining to observe the pathology of damaged tissue and enzyme-linked immunosorbent assay to detect changes in serum damage markers.