Beijing University of Chinese Medicine View Institution's Website 16 articles published in JoVE Medicine Synchronous Triplanar Reconstruction Integrated with Color Doppler Mapping for Precise and Rapid Localization of Thyroid Lesions Zhi Chen1, Zhiguo Ding1,2, Rui Hu1, Tengxiao Liang3, Fangliang Xing4, Shuo Qi1,2 1Department of Thyroid, Sunsimiao Hospital, Beijing University of Chinese Medicine, 2Department of Thyroid, Dongzhimen Hospital, Beijing University of Chinese Medicine, 3Fever Clinics, Dongzhimen Hospital, Beijing University of Chinese Medicine, 4Beijing Intelligent Entropy Science & Technology Co Ltd. Here we present a 5D ultrasound technique combining multi-planar 3D reconstruction and color Doppler fusion, which enables synchronous visualization of thyroid structural and functional information. By minimizing blind spots, this method allows rapid, precise localization of lesions to improve diagnostic accuracy, especially benefiting novice practitioners. Medicine A 3D Quantification Technique for Liver Fat Fraction Distribution Analysis Using Dixon Magnetic Resonance Imaging Fan Zhao1, Genming Zhang2, Zhongjian Tan3, Tengxiao Liang4, Fangliang Xing5 1Department of Infectious Diseases, Dongzhimen Hospital, Beijing University of Chinese Medicine, 2The First Clinical College, Beijing University of Chinese Medicine, 3Department of Radiology, Dongzhimen Hospital, Beijing University of Chinese Medicine, 4Fever Clinics, Dongzhimen Hospital, Beijing University of Chinese Medicine, 5Beijing Intelligent Entropy Science & Technology Co Ltd. This study introduces a unique 3D quantification method for liver fat fraction (LFF) distribution using Dixon Magnetic Resonance Imaging (Dixon MRI). LFF maps, derived from in-phase and water-phase images, are integrated with 3D liver contours to differentiate LFF patterns between normal and steatotic livers, enabling precise assessment of liver fat content. Medicine Application of Acupotomy in a Knee Osteoarthritis Model in Rabbit Xing LongFei1, Guo Yan2, Chen XiLin1, Hu TingYao1, Zhu WenTing3, Ma WeiWei1, Du Mei1, Xu Yue1, Guo ChangQing1 1School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, 2Acupuncture and Moxibustion Department, Beijing Hospital of Traditional Chinese Medicine affiliated with Capital Medical University, 3The Third Affiliated Hospital of Beijing University of Chinese Medicine In this protocol, a knee osteoarthritis model was prepared using the modified Videman method, and the operation procedures and precautions of acupotomy are detailed. The effectiveness of acupotomy has been demonstrated by testing the mechanical properties of quadriceps femoris and tendon and the mechanical and morphological properties of cartilage. Medicine Three-Dimensional Reconstruction for the Whole Lung with Early Multiple Pulmonary Nodules Junyi Shi1, Fangliang Xing2, Yang Liu3, Tengxiao Liang4 1Beijing University of Chinese Medicine, 2Beijing Intelligent Entropy Science & Technology Co Ltd., 3Center for Integrated Chinese and Western Medicine, Beijing You An Hospital, Capital Medical University, 4Fever Outpatient Clinic, Dongzhimen Hospital of Beijing University of Chinese Medicine This study introduces a three-dimensional (3D) reconstruction method for the entire lung in patients with early multiple pulmonary nodules. It offers a comprehensive visualization of nodule distribution and their interplay with lung tissue, simplifying the assessment of diagnosis and prognosis for these patients. Medicine Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy Jie Chen1, Yu-fei Li1, Yun-shu Zhang1, Tian-hui Du1, Yang Lu1, Xin-yi Li1, Shu-wen Guo1,2 1School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 2Fangshan Chinese Medicine Hospital, Beijing University of Chinese Medicine Based on in vitro experiments, this study revealed the mechanism of crocetin in repairing oxidative stress damage of cardiomyocytes by influencing mitophagy, in which the PINK1/Parkin signaling pathway plays an important role. Medicine A 3D Digital Model for the Diagnosis and Treatment of Pulmonary Nodules Jingwen Xue1, Fangliang Xing2, Yang Liu3, Tengxiao Liang4 1Beijing University of Chinese Medicine, 2Beijing Intelligent Entropy Science & Technology Co Ltd., 3Center for Integrated Chinese and Western Medicine, Beijing You An Hospital, Capital Medical University, 4Fever Outpatient Clinic, Dongzhimen Hospital of Beijing University of Chinese Medicine The objective of this study is to develop a novel 3D digital model of pulmonary nodules that serves as a communication bridge between physicians and patients and is also a cutting-edge tool for pre-diagnosis and prognostic evaluation. Medicine 3D Printing Model of a Patient's Specific Lumbar Vertebra Huizhong Bai1, Zhuoluo Zhou1, Gang Liu1, Shengyuan Jiang1, Yaqi Zhang1, Xinwei Zuo1, Fangliang Xing2, Lin Xu1, Li Wang3, Xiaohong Mu1 1Department of Orthopedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, 2Beijing Intelligent Entropy Science & Technology Co Ltd., 3Beijing An Yuan Quan Lv Medical Research Institute This study aims to create a 3D-printed model of a patient-specific lumbar vertebra, which contains both the vertebra and spinal nerve models fused from high-resolution computed tomography (HRCT) and MRI-Dixon data. Medicine Fu's Subcutaneous Needling for Knee Osteoarthritis Pain Po-En Chiu*1,2, Zhonghua Fu*3,4, Jian Sun4,5,6, Guan-Wei Jian7,8, Te-Mao Li9, Li-Wei Chou10,11,12 1Department of Chinese Medicine, Chang Bing Show Chwan Memorial Hospital, 2Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, 3Institute of Fu's Subcutaneous Needling, Beijing University of Chinese Medicine, 4Clinical Medical College of Acupuncture & Moxibustion and Rehabilitation, Guangzhou University of Chinese Medicine, 5Second Clinical Medical College, Guangzhou University of Chinese Medicine, 6Guangdong Provincial Hospital of Chinese Medicine, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 7Graduate Institute of Acupuncture Science, China Medical University, 8Department of Chinese Medicine, Sinying Hospital, Ministry of Health and Welfare, 9School of Chinese Medicine, College of Chinese Medicine, China Medical University, 10Department of Physical Medicine and Rehabilitation, China Medical University Hospital, 11Department of Physical Therapy and Graduate Institute of Rehabilitation Science, China Medical University, 12Department of Physical Medicine and Rehabilitation, Asia University Hospital, Asia University We present a protocol for using Fu's subcutaneous needling for knee osteoarthritis pain, which combines swaying movement and reperfusion approach techniques. This protocol has great potential for future applications in myofascial pain treatment and could enhance Fu's subcutaneous needling (FSN) manipulation skills. Biochemistry Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds Yue Zhang*1, Peng Cao*2, Fang Lu1, Jinjun Cheng3, Huihua Qu4 1School of Life Science, Beijing University of Chinese Medicine, 2Third Affiliated Hospital, Beijing University of Chinese Medicine, 3National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, 4Center of Scientific Experiment, Beijing University of Chinese Medicine Biology Detecting Migration and Infiltration of Neutrophils in Mice Qingyi Lu*1, Kai Yuan*1, Xiaohong Li1, Haixu Jiang1, Guiyang Huo1, Wenrui Jia1, Guangrui Huang1, Anlong Xu1,2 1School of Life Sciences, Beijing University of Chinese Medicine, 2State Key Laboratory of Biocontrol, Department of Biochemistry, School of Life Sciences, Sun Yat-Sen (Zhongshan) University Here, we present three methods to assess neutrophil migration and infiltration both in vivo and in vitro. These methods can be used to discover promising therapeutics targeting neutrophil migration. Behavior Analysis of Learning and Memory Ability in an Alzheimer's Disease Mouse Model using the Morris Water Maze Huiling Tian1, Ning Ding2, Mengwei Guo1, Shun Wang1, Zidong Wang1, Hao Liu1, Jiayi Yang1, Yujie Li3, Jingyu Ren1, Jing Jiang4, Zhigang Li1 1School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, 2Guang'anmen Hospital of China Academy of Chinese Medical Science, 3The Third Affiliated Hospital of Beijng University of Chinese Medicine, 4School of Nursing, Beijing University of Chinese Medicine Herein, a protocol to conduct the Morris water maze tests to evaluate the ability of learning and memory of Alzheimer’s Disease model mice and to assess the effect of manual acupuncture for treating them is described. Immunology and Infection Generation of Monoclonal Antibodies Against Natural Products Yue Zhang1, Peng Cao2, Fang Lu3, Xin Yan4, Bingqian Jiang3, Jinjun Cheng3, Huihua Qu5 1School of Life Science, Beijing University of Chinese Medicine, 2Third Affiliated Hospital, Beijing University of Chinese Medicine, 3School of Basic Medical Sciences, Beijing University of Chinese Medicine, 4School of Chinese Materia Medica, Beijing University of Chinese Medicine, 5Center of Scientific Experiment, Beijing University of Chinese Medicine This article provides a detailed protocol for the preparation and evaluation of monoclonal antibodies against natural products for use in various immunoassays. This procedure includes immunization, cell fusion, indirect competitive ELISA for positive clone screening, and monoclonal hybridoma preparation. The specifications for antibody characterization using MALDI-TOF-MS and ELISA analyses are also provided. Behavior Evaluating the Anti-depression Effect of Xiaoyaosan on Chronically-stressed Mice Zhi-Yi Yan*1, Xiao-Juan Li*1,2, Xiu-Fang Ding1, Yue-Yun Liu1, Jia-xu Chen1,2 1School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 2Formula-pattern Research Center, School of Traditional Chinese Medicine, Jinan University Here, we present a protocol to establish the mouse depressive model, observe the behavior changes associated with chronic unpredictable mild stress (CUMS), and evaluate the anti-depression effect of Xiaoyaosan. Neuroscience A Rat Model of Central Fatigue Using a Modified Multiple Platform Method Weiyue Zhang*1, Wei Zhang*1, Ning Dai*1, Chenxia Han2, Fengzhi Wu1, Xu Wang1, Libo Tan1, Jie Li1, Feng Li1, Qingjia Ren3 1School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 2Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, 3Institute of Tibetan Medicine, Tibetan Traditional Medical College Here, we present a protocol to introduce a rat model of central fatigue using the modified multiple platform method (MMPM). Medicine A Doxorubicin-induced Cardiomyopathy Model in Adult Zebrafish Xiao Ma*1,2,3, Yonghe Ding*2,3, Yong Wang2,3,4, Xiaolei Xu1,2,3 1Clinical and Translational Sciences Track, Mayo Clinic Graduate School of Biomedical Sciences, 2Department of Biochemistry and Molecular Biology, Mayo Clinic, 3Division of Cardiovascular Diseases, Mayo Clinic, 4Institute of Life Science, Beijing University of Chinese Medicine A method to generate a doxorubicin-induced cardiomyopathy model in adult zebrafish (Danio rerio) is described here. Two alternative ways of intraperitoneal injection are presented and conditions to reduce variations among different experimental groups are discussed. Biology Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure Kenneth K. Kwong1,2, Lenuta Kloetzer1,2,3,4, Kelvin K. Wong5,6, Jia-Qian Ren1,2, Braden Kuo1,2,3,4, Yan Jiang7, Y. Iris Chen1,2, Suk-Tak Chan1,2,8, Geoffrey S. Young9, Stephen T.C. Wong5,6 1Department of Radiology, Massachusetts General Hospital, Harvard Medical School, 2Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, 3Gastrointestinal Unit, Massachusetts General Hospital, Harvard Medical School, 4Department of Medicine, Massachusetts General Hospital, Harvard Medical School, 5Center for biotechnology and Informatics, The Methodist Hospital Research Institute, 6Department of Radiology, The Methodist Hospital, Weill Cornell Medical College, 7Bejing University of Chinese Medicine, 8Department of Health Technology and Informatics, The Hong Kong Polytechnic University, 9Department of Radiology, Brigham and Women's Hospital, Harvard Medical School Gua Sha, traditional Chinese therapeutic skin scraping, causes subcutaneous microvascular blood extravasation. We report a protocol of bioluminescence imaging of HO-1-luciferase transgenic mice to demonstrate that Gua Sha upregulates heme oxygenase-1 (HO-1) in multiple organs.
