University of Science and Technology of China View Institution's Website 10 articles published in JoVE Immunology and Infection Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice Meng Wang*1, Yuanyuan Huang*1, Wangpeng Gu2,3, Haikun Wang1 1CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, University of Chinese Academy of Sciences, 2School of Life Science, University of Science and Technology of China, 3State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences This paper describes protocols of evaluating Tfh and GC B response in mouse model of influenza virus infection. Medicine Spinal Cord Lateral Hemisection and Asymmetric Behavioral Assessments in Adult Rats Xiao-Jing Lin1,2,3, Shaonan Wen2,6, Ling-Xiao Deng3, Heqiao Dai3, Xiaolong Du3, Chen Chen3,4, Melissa J. Walker3,4, Ting-Bao Zhao1,5, Xiao-Ming Xu3 1Department of Spinal Cord Injury and Repair, Trauma and Orthopedics Institute of Chinese PLA, 960th Hospital, Joint Logistics Support Force of PLA, 2Institute of Military Cognition and Brain Sciences, Academy of Military Medical Sciences, Academy of Military Sciences, 3Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Department of Neurological Surgery and Goodman Campbell Brain and Spine, Indiana University School of Medicine, 4Program in Medical Neuroscience, Paul and Carole Stark Neurosciences Research Institute, Indiana University School of Medicine, 5Department of Orthopedics, the Third Affiliated Hospital, Shandong University, 6Beijing Computing Center, Beijing Academy of Science and Technology Here we describe surgical procedures to produce a reliable spinal cord lateral hemisection (HX) at the 9th thoracic level in adult rats and neurobehavioral assessments designed for detecting asymmetric deficits after such a unilateral injury. Bioengineering Optical Imaging of Isolated Murine Ventricular Myocytes Shuxin Han1,2, Matt Klos3, Sherry Morgenstern3, Ramiz Ahmad3, Isabella Pua3, Shreyas Suresh1, Kayla Hicks3, Eric Devaney3 1Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China, 2Central Nodal (Anhui) Bioscience and Technology Research Center, Hefei, Anhui, China, 3Pediatric Cardiac and Thoracic Surgery, University Hospitals Cleveland Medical Center We present the methodology for the isolation of murine myocytes and how to obtain voltage or calcium traces simultaneously with sarcomere shortening traces using fluorescence photometry with simultaneous digital cell geometry measurements. Engineering Multimodal 3D Printing of Phantoms to Simulate Biological Tissue Canzhen Ma*1,2, Shuwei Shen*1,2, Guangli Liu1, Siyue Guo1,2, Buyun Guo1,2, Jialuo Li1,2, Kuiming Huang1,2, Yidan Zheng1, Pengfei Shao1,2, Erbao Dong1,2, Jiaru Chu1, Ronald X. Xu1,2,3 1Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, 2Key Laboratory of Precision Scientific Instrumentation of Anhui Higher Education Institutes, University of Science and Technology of China, 3Department of Biomedical Engineering, The Ohio State University Spin coating, polyjet printing, and fused deposition modeling are integrated to produce multilayered heterogeneous phantoms that simulate structural and functional properties of biological tissue. Developmental Biology An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity Mengrou Liu*1, Chunsen Zhu*1, Shun Bai*2, Xin Li1, Kaiqiang Fu1, Lan Ye1, Ke Zheng1 1State Key Laboratory of Reproductive Medicine, Nanjing Medical University, 2Center for Reproductive Medicine, Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China Here, we present a protocol to assess the blood-testis barrier integrity by injecting inulin-FITC into testes. This is an efficient in vivo method to study blood-testis barrier integrity that can be compromised by genetic and environmental elements. Behavior Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila Lifen Jiang*1,2, Yinpeng Zhan*2,3, Yan Zhu2,3 1School of Life Science, University of Science and Technology of China, 2State key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, 3University of Chinese Academy of Sciences Quantitative food-intake assays with dyed food provide a robust and high-throughput means to evaluate feeding motivation. Combining the food consumption assay with thermogenetic and optogenetic screens is a powerful approach to investigate the neural circuits underlying appetite in adult Drosophila melanogaster. Immunology and Infection An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood Ke Yang*1,2,3, Jiandong Wu*3,4, Ling Zhu1, Yong Liu1, Michael Zhang5, Francis Lin3,4,6,7 1Institute of Applied Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, 2University of Science and Technology of China, 3Department of Physics and Astronomy, University of Manitoba, 4Department of Biosystems Engineering, University of Manitoba, 5Seven Oaks General Hospital, 6Department of Immunology, University of Manitoba, 7Department of Biological Sciences, University of Manitoba This article provides the detailed method of performing a rapid neutrophil chemotaxis assay by integrating the on-chip neutrophil isolation from whole blood and the chemotaxis test on a single microfluidic chip. Neuroscience Retrograde Labeling of Retinal Ganglion Cells in Adult Zebrafish with Fluorescent Dyes Su-Qi Zou1, Chen Tian1, Su-Tie Du1, Bing Hu1 1Laboratory of Neurodevelopement and Repair, University of Science and Technology of China We introduce an efficient method to retrograde label retinal ganglion cells (RGCs) in adult zebrafish. Engineering Evaluating Plasmonic Transport in Current-carrying Silver Nanowires Mingxia Song1, Arnaud Stolz1, Douguo Zhang2, Juan Arocas1, Laurent Markey1, Gérard Colas des Francs1, Erik Dujardin3, Alexandre Bouhelier1 1Laboratoire Interdisciplinaire Carnot de Bourgogne CNRS-UMR 6303, Université de Bourgogne, 2Department of Optics and Optical Engineering, University of Science and Technology of China, 3CEMES, CNRS-UPR 8011 Silver nanowires can simultaneously transport electrons and optical information in the form of surface plasmons. A procedure is described here to realize such a shared circuitry and the limitations at propagating both information carriers are evaluated. Biology Using the optokinetic response to study visual function of zebrafish Su-Qi Zou1, Wu Yin1, Ming-Jing Zhang1, Chun-Rui Hu1, Yu-Bin Huang1, Bing Hu1 1Laboratory of Neurodevelopment and Repair, Department of Neurobiology and Biophysics, School of Life, University of Science and Technology of China (USTC) Optokinetic response has been widely used to assess the visual functions of larval zebrafish. Nevertheless, the standard protocol for larval fish is not yet readily applicable in adults1-5. Here, we introduce how to measure the OKR of adult zebrafish using a new protocol which is established in our lab.