ARO-Newe Ya‘ar Center 1 article published in JoVE Genetics Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa Shahar Cohen1, Maxim Itkin1, Adi Faigenboim1, Rachel Davidovich-Rikanati2, Einat Bar2, Daniel Hasson1, Nurit Shalev3, Hinanit Koltai3, Oded Sagee4, Efraim Lewinsohn2, Ben Spitzer-Rimon3, Arthur A. Schaffer1 1Department of Vegetable Research, ARO-Volcani Center, 2Department of Vegetable Research, ARO-Newe Ya‘ar Center, 3Department of Ornamental Horticulture and Biotechnology, Institute of Plant Sciences, ARO- Volcani Center, 4Department of Fruit Science, ARO- Volcani Center A protocol is presented for the convenient and high-throughput isolation and enrichment of glandular capitate stalked and sessile trichomes from Cannabis sativa. The protocol is based on a dry, non-buffer extraction of trichomes using only liquid nitrogen, dry ice, and nylon sieves and is suitable for RNA extraction and transcriptomic analysis.