Pittsburg State University 1 article published in JoVE Immunology and Infection Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7 Tyler Shelby1, Shoukath Sulthana1, James McAfee1, Tuhina Banerjee1, Santimukul Santra1 1Department of Chemistry and Kansas Polymer Research Center, Pittsburg State University The overall goal of this protocol is to synthesize functional nanosensors for the portable, cost-effective, and rapid detection of specifically targeted pathogenic bacteria through a combination of magnetic relaxation and fluorescence emission modalities.