Crop Improvement and Genetics Research Unit 1 article published in JoVE Biology mirMachine: A One-Stop Shop for Plant miRNA Annotation H. Busra Cagirici1, Taner Z. Sen1, Hikmet Budak2 1U.S. Department of Agriculture - Agricultural Research Service, Western Regional Research Center, Crop Improvement and Genetics Research Unit, CA, USA, 2Montana BioAgriculture Inc., Missoula, MT, USA Herein, we present a new and fully automated miRNA pipeline, mirMachine that 1) can identify known and novel miRNAs more accurately and 2) is fully automated and freely available. Users can now execute a short submission script to run the fully automated mirMachine pipeline.