Universidade Federal do Rio Grande do Sul View Institution's Website 4 articles published in JoVE Neuroscience Using Home-based, Remotely Supervised, Transcranial Direct Current Stimulation for Phantom Limb Pain Kevin Pacheco-Barrios*1,2, Daniela Martinez-Magallanes*1, Cristina Xicota Naqui1,3, Marianna Daibes1, Elly Pichardo1, Alejandra Cardenas-Rojas1, David Crandell4, Anahita Dua5, Abhishek Datta6,7, Wolnei Caumo8,9,10, Felipe Fregni1 1Neuromodulation Center and Center for Clinical Research Learning, Spaulding Rehabilitation Hospital, Massachusetts General Hospital, Harvard Medical School, 2Vicerrectorado de Investigación, Unidad de Investigación para la Generación y Síntesis de Evidencias en Salud, Universidad San Ignacio de Loyola, 3Nursing Department, Universitat Internacional de Catalunya, 4Spaulding Rehabilitation Hospital, Harvard Medical School, 5Division of Vascular and Endovascular Surgery, Massachusetts General Hospital, Harvard Medical School, 6Research and Development, Soterix Medical, 7City College of New York, 8Post-Graduate Program in Medical Sciences, School of Medicine, Universidade Federal do Rio Grande do Sul (UFRGS), 9Laboratory of Pain & Neuromodulation, Hospital de Clinicas de Porto Alegre (HCPA), 10Pain and Palliative Care Service, Hospital de Clinicas de Porto Alegre (HCPA) The goal of this study is to describe a protocol for the home-based delivery of remotely supervised transcranial direct current stimulation (RS-tDCS) conserving the standard procedures of in-clinic practice, including safety, reproducibility, and tolerability. The participants included will be patients with phantom limb pain (PLP). Medicine Home-Based Transcranial Direct Current Stimulation Device Development: An Updated Protocol Used at Home in Healthy Subjects and Fibromyalgia Patients Fabiana Carvalho1,2, Aline Patrícia Brietzke1,2, Assunta Gasparin1,2, Franciele Pereira dos Santos2,3, Rafael Vercelino4, Rafael Firmino Ballester2,3, Paulo Roberto Stefani Sanches5, Danton Pereira da Silva Jr5, Iraci L. S. Torres1,6, Felipe Fregni7,8, Wolnei Caumo1,2,9,10 1Post-Graduate Program in Medical Sciences, School of Medicine, Universidade Federal do Rio Grande do Sul (UFRGS), 2Laboratory of Pain & Neuromodulation, Hospital de Clínicas de Porto Alegre (HCPA), 3School of Medicine, Universidade Federal do Rio Grande do Sul (UFRGS), 4Faculdade de Desenvolvimento do Rio Grande do Sul (FADERGS), Health and Wellness School Laureate International Universities, 5Biomedical Engineering Department, Hospital de Clínicas de Porto Alegre (HCPA), 6Pharmacology Department, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), 7Department of Physical Medicine and Rehabilitation, Harvard Medical School, 8Spaulding Neuromodulation Center, Spaulding Rehabilitation Hospital, 9Pain and Palliative Care Service, Hospital de Clínicas de Porto Alegre (HCPA), 10Department of Surgery, School of Medicine, Universidade Federal do Rio Grande do Sul (UFRGS) This study provides an updated home-based tDCS protocol that enables subjects to receive the beneficial effects of tDCS at home with an easy to use device with settings to control the use and dosage, enhancing the feasibility for long-term use at home. Behavior Simultaneous EEG Monitoring During Transcranial Direct Current Stimulation Pedro Schestatsky1,2,3, Leon Morales-Quezada3,4, Felipe Fregni3 1Programa de Pós-Graduação em Ciências Médica, Universidade Federal do Rio Grande do Sul, 2Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), 3Laboratory of Neuromodulation, Department of Physical Medicine & Rehabilitation, Spaulding Rehabilitation Hospital and Massachusetts General Hospital, Harvard Medical School, 4De Montfort University Transcranial direct current stimulation (tDCS) is a non-invasive brain stimulation technique that has shown initial therapeutic effects in several neurological conditions. The main mechanism underlying these therapeutic effects is the modulation of cortical excitability. Therefore, online monitoring of cortical excitability would help guide stimulation parameters and optimize its therapeutic effects. In the present article we review the use of a novel device that combines simultaneous tDCS and EEG monitoring in real time. Biology Blood Collection for Biochemical Analysis in Adult Zebrafish Gabriela L. Pedroso1,2, Thais O. Hammes1,2, Thayssa D.C. Escobar1, Laisa B. Fracasso1, Luiz Felipe Forgiarini1, Themis R. da Silveira1,2 1Hospital de Clínicas de Porto Alegre, Centro de Pesquisa Experimental Laboratório de Hepatologia e Gastroenterologia Experimental, 2Universidade Federal do Rio Grande do Sul, UFRGS. Porto Alegre, RS, Brasil This paper presents a technique for collection of blood from the dorsal aorta of Zebrafish. It also provides instructions for obtaining serum for use in biochemical analyses, such as tests for determining cholesterol and triglyceride levels.
