JoVE Journal
Neuroscience
Neuroscience
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
We describe how to implement photoactivated localization microscopy (PALM)-based studies of vesicles in fixed, cultured neurons. Key components of our protocol include labeling vesicles with photoconvertible chimeras, collecting sparsely sampled raw images with a super-resolution microscopy system, and processing the raw images to produce a super-resolution image.