JoVE Journal
Bioengineering
Bioengineering
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
We present a protocol for immobilizing single macromolecules in microfluidic devices and quantifying changes in their conformations under shear flow. This protocol is useful for characterizing the biomechanical and functional properties of biomolecules such as proteins and DNA in a flow environment.