JoVE Journal
Bioengineering
Bioengineering
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
This protocol describes techniques for isolating primary mouse hepatocytes from the liver and electroporating CRISPR-Cas9 as ribonucleoproteins and mRNA to disrupt a therapeutic target gene associated with an inherited metabolic disease of the liver. The methods described result in high viability and high levels of gene modification after electroporation.