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Journal / Genetics / Inmunofluorescencia dual de γH2AX y 53BP1 en…
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Genetics
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JoVE Journal Genetics
Inmunofluorescencia dual de γH2AX y 53BP1 en linfocitos periféricos humanos
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Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds
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Inmunofluorescencia dual de γH2AX y 53BP1 en linfocitos periféricos humanos

Article DOI: 10.3791/65472-v • 05:34 min • July 14th, 2023
July 14th, 2023 •
1, 1,2, 1, 1
1Unità di Genetica, Dipartimento di Biologia, University of Pisa, 2Department of Women-Child-Newborn Obstetrics and Gynaecology, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico

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Este protocolo presenta un método para evaluar la formación y reparación de roturas de doble cadena de ADN mediante la detección simultánea de focos γH2AX y 53BP1 en núcleos interfase de linfocitos periféricos humanos tratados con bleomicina.

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Este mes en JoVE Número 197
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