Journal of Visualized Experiments (JoVE) is a video journal for biological research. |
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03/10/2010
Werner Arber
Swiss microbial geneticist, Werner Arber shared the 1978 Nobel Prize in Physiology or Medicine with Hamilton Smith and Daniel Nathans for their discovery of restriction endonucleases. Arber found that viral DNA introduced into a non-specific bacterial host was changed, while host DNA was protected by methylation. He theorized that a microbial enzyme cut the DNA into smaller pieces, while at the same time, the methylated host DNA was protected from its own enzymes. Work done by Nathans and Smith later showed his theory was later shown to be correct. This work laid the foundation for recombinant DNA technology.
03/09/2010
Stephanie A. Zlatic1, Pearl V. Ryder1, Gloria Salazar2, Victor Faundez1
1Department of Cell Biology, Emory University, 2Department of Medicine, Division of Cardiology, Emory University
The cell permeable crosslinker DSP [dithiobis-(succinimidyl propionate)] stabilizes transient and labile interactions in vivo, which allows their isolation using stringent protein complex purification techniques. Here we present a technique for crosslinking cells grown in culture followed by isolation of protein complexes by immunoprecipitation.
03/08/2010
Virginia M. Hazen1, 2,*, Keith Phan1, Ken Yamauchi1, 2,*, Samantha J. Butler1, 2,*
1Department of Biological Sciences, University of Southern California, 2Neuroscience Graduate Program, University of Southern California
* These authors contributed equally
This assay assesses the ability of a signaling molecule, here Bone Morphogenetic Protein 7 (BMP7), to reorient commissural axons. An explant of embryonic dorsal spinal cord is cultured adjacent to an aggregate of COS cells secreting the candidate growth factors. Reoriented commissural axons growing within the explant are visualized by immunohistochemistry.
03/05/2010
Tanja M. Liebig, Anne Fiedler, Nela Klein-Gonzalez, Alexander Shimabukuro-Vornhagen, Michael von Bergwelt-Baildon
Laboratory for Tumor and Transplantation Immunology, Department I of Internal Medicine, University Hospital of Cologne
In this video, we demonstrate the procedure of CD40-activation and expansion of murine B cells from splenocytes of C57BL/6 mice, which can be used as a model antigen-presenting cell (APC) to study induction of immunity.
03/04/2010
Tripti Gupta, Mary C. Mullins
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine
This protocol describes a procedure for identifying and dissecting organs from the adult zebrafish.
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