The Eighth Affiliated Hospital of Sun Yat-sen University 2 articles published in JoVE Medicine Fluorescent Laparoscopic Central Hepatectomy for Liver Cancer Using Indocyanine Green Negative Staining Jinxing Wei*1, Haixiong Zhang*2, Jinyi Zhong1, Jiyan Xie3, Weibang Yang4, Jinxin Duan1, Wenfeng Zhuo1, Ang Li1, Changzhen Shang1, Jun Min1 1Department of Hepatobiliary Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 2Department of Hepatobiliary Surgery, Foshan Fosun Chancheng Hospital, 3Department of Gastrointestinal Surgery, The Eighth Affiliated Hospital of Sun Yat-sen University, 4Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Guangzhou Medical University The present protocol describes fluorescent negative staining in laparoscopic central hepatectomy. This technique can make hepatectomy more accurate and precise. Developmental Biology A Mini-Invasive Internal Fixation Technique for Studying Immobilization-Induced Knee Flexion Contracture in Rats Shihai Jiang*1, Xiaoyou Yi*3, Yuansen Luo2, Dongjie Yu1, Yuangao Liu1, Fei Zhang1,4, Lei Zhu2, Kun Wang1 1Department of Joint and Trauma Surgery, The Third Affiliated Hospital of Sun Yat-sen University, 2Department of Plastic Surgery, The Third Affiliated Hospital of Sun Yat-sen University, 3Department of Bone Surgery, Tungwah Hospital of Sun Yat-sen University, 4Department of Othopaedic Surgery, The Eighth Affiliated Hospital of Sun Yat-sen University Here, we present a protocol to describe a minimally invasive technique for knee joint immobilization in a rat model. This reproducible protocol, basing on muscle-gap separation modus and the mini-incision skill, is suitable for studying the underlying molecular mechanism of acquired joint contracture.