Hangzhou Medical College 3 articles published in JoVE Biology Hand-Rearing Method for Infant Marmosets Hao Sun1,2, Rui Li1, Yingxu Lin1,2, Xinyuan Cao1, Lingzhu Fan1, Guanglong Sun1, Min Xie1, Lin Zhu1, Chencen Yu1, Ruolan Cai1, Chenfei Lyu1, Xiaohui Wang1,2, Yuanqing Zhang1,2, Siyi Bai1,2, Runze Qi1, Binliang Tang1,3, Guoqiang Jia1,4, Xinjian Li1,2,4, Lixia Gao1,2,4 1Department of Neurology of the Second Affiliated Hospital and Interdisciplinary Institute of Neuroscience and Technology, School of Medicine, Zhejiang University, 2Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, 3Rehabilitation Medicine Center, Rehabilitation & Sports Medicine Research Institute of Zhejiang Province, Department of Rehabilitation Medicine, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital, Hangzhou Medical College, 4NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Science and Brain-Machine Integration, School of Brain Science and Brain Medicine, Zhejiang University Here, we describe a hand-rearing method for raising infant marmosets in an animal incubator. This method greatly increases the survival rate of marmoset infants, which provides the opportunity to study the development of marmoset infants with similar genetic backgrounds raised in different postnatal environments. Neuroscience Ultrasound-Guided Needle Release Combined with Corticosteroid Injection for the Treatment of Supinator Syndrome Zeng Zeng1,2, Cong-Xian Chen1 1Cancer Center, Department of Ultrasound Medicine, Zhejiang Provincial People’s Hospital, Affiliated People’s Hospital, Hangzhou Medical College, 2Zhejiang University School of Medicine The deep branch of the radial nerve can easily be compressed at the arcade of Frohse due to its anatomical features. Ultrasound-guided needle release combined with corticosteroid injection is an effective and safe treatment for deep branch radial nerve adhesion. Bioengineering Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632 Zhiwei Xie1,2,3, Jizhou Shi4, Min Zong3, Qiuping Xu1, Chang Liu1, Jie Wen1, Qun Zhang1, Panpan Liu1, Guanyi Liu1, Jing Guo2,5, Xunwei Wu1 1Department of Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 2Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 3Department of Stomatology, Shengli Oilfield Central Hospital, 4Department of Pediatric Surgery, Shengli Oilfield Central Hospital, 5Ningbo Stomatology Hospital of Savaid Stomatology School, Hangzhou Medical College Here we present a modified method for the isolation and culture of human gingival epithelial cells by adding the Rock inhibitor, Y-27632, to the traditional method. This method is easier, less time-consuming, enhances stem cell properties, and produces larger numbers of high-potential epithelial cells both for the laboratory and for clinical applications.