Zhejiang Cancer Hospital 2 articles published in JoVE Biology A Magnetic Separation-Assisted High-Speed Homogenization Method for Large-Scale Production of Endosome-Derived Vesicles Dujuan Wang1,2, Shili Yao2,3, Peng Guo1,2,3,4 1Academy of Medical Engineering and Translational Medicine, Tianjin University, 2Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, 3School of Materials Science and Engineering, Tianjin University, 4Zhejiang Cancer Hospital Here, we describe a magnetic separation-assisted high-speed homogenization method for large-scale production of endosome-derived nanovesicles as a new type of exosome mimics (EMs) that share the same biological origin and similar structure, morphology, and protein composition of native extracellular vesicles (EVs). Medicine Establishment of a Simple and Effective Rat Model for Intraoperative Parathyroid Gland Imaging Fan Chen*1,2, Chun Liu*3, Peng Guo3, Weihui Zheng1,2 1Key Laboratory of Head & Neck Cancer Translational Research of Zhejiang Province, Zhejiang Cancer Hospital, 2The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), 3Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences To date, the development of parathyroid gland (PG) identification methods is limited by the lack of animal models in preclinical research. Here, we establish a simple and effective rat model for intraoperative PG imaging and evaluate its effectiveness by using iron oxide nanoparticles as a novel PG contrast agent.