Chungbuk National University View Institution's Website 5 articles published in JoVE Chemistry Synthesis of Triazole and Tetrazole-Functionalized Zr-Based Metal-Organic Frameworks Through Post-Synthetic Ligand Exchange Sangho Lee*1, Daeyeon Lee*1, Jin Yeong Kim2, Min Kim1 1Department of Chemistry, Chungbuk National University, 2Department of Chemistry Education, Seoul National University Post-synthetic ligand exchange (PSE) is a versatile and powerful tool for installing functional groups into metal-organic frameworks (MOFs). Exposing MOFs to solutions containing triazole- and tetrazole-functionalized ligands can incorporate these heterocyclic moieties into Zr-MOFs through PSE processes. Chemistry Development of Heterogeneous Enantioselective Catalysts using Chiral Metal-Organic Frameworks (MOFs) Jeehwan Han*1, Seongwoo Kim*2, Mi Sun Lee1, Min Kim2, Nakcheol Jeong1 1Department of Chemistry, Korea University, 2Department of Chemistry and BK21Plus Research Team, Chungbuk National University Here, we present a protocol for active site validation of metal-organic framework catalysts by comparing stoichiometric and catalytic carbonyl-ene reactions to find out whether a reaction takes place on the inner or outer surface of metal-organic frameworks. Bioengineering A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons Se Hee Lee*1, Ho Won Lee*2, Da Som Kim2, Hyuck Gi Kwon3, Jong Hyun Lee3, Yang-Hoon Kim1, Ok Chan Jeong2,3, Ji-Young Ahn1 1College of Natural Sciences, Chungbuk National University, 2Department of Biomedical Engineering, Inje University, 3Institute of Digital Anti-Aging Healthcare, Inje University This work provides a method for the fabrication of droplet-based microfluidic platforms and the application of polyacrylamide microspheres for microsphere-PCR amplification. The microsphere-PCR method makes it possible to obtain single-stranded DNA amplicons without separating double-stranded DNA. Immunology and Infection Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation Jun-O Jin1,2, Soyeong Jang3, Hyehyun Kim4, Junghwan Oh4, Sungbo Shim5, Minseok Kwak3,4, Peter C.W. Lee6 1Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, 2Department of Medical Biotechnology, Yeungnam University, 3Department of Chemistry, Pukyong National University, 4Marine-integrated Bionics Research Center, Pukyong National University, 5Department of Biochemistry, Chungbuk National University, 6Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center Experimental procedures for the subsequent extraction of lymphatic tissues to test lymphoid dendritic cell activation are described after treatment of an immunostimulating nanomaterial. Bioengineering Fluorescence detection methods for microfluidic droplet platforms Xavier Casadevall i Solvas1, Xize Niu1, Katherine Leeper1, Soongwon Cho1, Soo-Ik Chang2, Joshua B. Edel1, Andrew J. deMello3 1Department of Chemistry, Imperial College London, 2Department of Biochemistry, Protein Chip Research Center, Chungbuk National University, 3Department of Chemistry and Applied Biosciences, Institute for Chemical and Bioengineering, ETH Zurich Droplet-based microfluidic platforms are promising candidates for high throughput experimentation since they are able to generate picoliter, self-compartmentalized vessels inexpensively at kHz rates. Through integration with fast, sensitive and high resolution fluorescence spectroscopic methods, the large amounts of information generated within these systems can be efficiently extracted, harnessed and utilized.