The Centre for Regenerative Medicine 1 article published in JoVE Immunology and Infection Interrogating Cell-Cell Interactions in the Salivary Gland via Ex Vivo Live Cell Imaging Sonia Elder1, Justyna Cholewa-Waclaw1, Elaine Emmerson1 1The Centre for Regenerative Medicine, Institute for Regeneration and Repair, The University of Edinburgh Immunofluorescent imaging is constrained by the ability to observe complex, time-dependent biological processes in just a single snapshot in time. This study outlines a live-imaging approach conducted on precision-cut mouse submandibular gland slices. This approach allows for the real-time observation of cell-cell interactions during homeostasis and the processes of regeneration and repair.