University and Hospital Trust of Verona 5 articles published in JoVE Medicine Robotic D3 Partial Duodenal Resection with Primary Side-to-Side Anastomosis Roberto Maria Montorsi1,2,3, Sofia Xenaki1,2,4, Sebastiaan Festen5, Paul Fockens2,6, Barbara A. J. Bastiaansen2,6, Freek Daams2,7, Olivier R. Busch1,2, M. G. Besselink1,2, HPB-Amsterdam, 1Amsterdam UMC, Department of Surgery, University of Amsterdam, 2Cancer Center Amsterdam, 3Department of General and Pancreatic Surgery, The Pancreas Institute, University of Verona Hospital Trust, 4Department of General Surgery, University Hospital of Heraklion Crete, 5Department of Surgery, OLVG, 6Amsterdam UMC, Department of Gastroenterology, University of Amsterdam, 7Amsterdam UMC, Department of Surgery, Vrije Universiteit This protocol presents a case of a robotic partial duodenal resection with primary side-to-side duodeno-jejunal reconstruction in a patient with a 5 cm duodenal stenosis. This is done at the third duodenal segment (D3) after an endoscopic mucosal resection (EMR) for a duodenal polyp. Medicine Laparoscopic Radical Left Pancreatectomy for Pancreatic Cancer: Surgical Strategy and Technique Video Frederique L. Vissers1, Maurice J.W. Zwart1, Alberto Balduzzi1,3, Maarten Korrel1, Sanne Lof2, Mohammad Abu Hilal*2, Marc G. Besselink*1 1Department of Surgery, Cancer Center Amsterdam, Amsterdam UMC, University of Amsterdam, 2Department of Surgery, Southampton University Hospital NHS Foundation Trust, 3General and Pancreatic Surgery Department, Pancreas Institute, University and Hospital Trust of Verona Oncologically safe left pancreatectomy requires radical resection (R0), Gerota’s (perirenal) fascia resection, and adequate lymph node dissection. This study describes the technical details of laparoscopic radical left pancreatectomy (LRLP), used in the first international multicenter randomized trial comparing minimally invasive with open left pancreatectomy for pancreatic cancer, the DIPLOMA trial. Medicine Robotic Lateral Pancreaticojejunostomy for Chronic Pancreatitis Alberto Balduzzi*1,2, Maurice J. W. Zwart*1, Rens M. A. Kempeneers1, Marja A. Boermeester1, Olivier R. Busch1, Marc G. Besselink1 1Department of Surgery, Amsterdam Gastroenterology and Metabolism, Amsterdam UMC, University of Amsterdam, 2General and Pancreatic Surgery Department, Pancreas Institute, University and Hospital Trust of Verona Robotic lateral pancreaticojejunostomy (RLPJ) may be used in patients with painful, morphine dependent, chronic pancreatitis and a dilated main pancreatic duct. We describe a standardized and reproducible technique for RLPJ, which includes transection of the gastroduodenal artery. Behavior Comparative Lesions Analysis Through a Targeted Sequencing Approach Caterina Vicentini1, Andrea Mafficini1, Michele Simbolo2, Matteo Fassan3, Pietro Delfino2, Rita Teresa Lawlor1, Borislav Rusev1, Aldo Scarpa1,2, Vincenzo Corbo1,2 1ARC-Net Research Centre, University and Hospital Trust of Verona, 2Department of Diagnostics and Public Health, Section of Pathology, University and Hospital Trust of Verona, 3Department of Medicine (DIMED), Surgical Pathology and Cytopathology Unit, University of Padua This article describes a method to identify clonal and subclonal alterations among different specimens from a given patient. Although the experiments described here focus on a specific tumor type, the approach is broadly applicable to other solid tumors. Cancer Research Laparoscopic Pancreatoduodenectomy With Modified Blumgart Pancreaticojejunostomy Matteo De Pastena1,2, Jony van Hilst1, Thijs de Rooij1, Olivier R Busch1, Michael F Gerhards3, Sebastiaan Festen3, Marc G Besselink1 1Department of Surgery, Cancer Center Amsterdam, Academic Medical Center, 2General and Pancreatic Surgery Department, Pancreas Institute, University and Hospital Trust of Verona, 3Department of Surgery, OLVG Laparoscopic pancreatoduodenctomy (LPD) may offer advantages over open pancreatoduodenectomy, including early postoperative mobilization, less delayed gastric emptying and a shorter hospital stay. However, LPD is technically challenging and not well-standardized, especially regarding the pancreatic anastomosis. We describe a standardized technique for the pancreatic anastomosis during LPD: modified Blumgart pancreaticojejunostomy.