University of Amsterdam 20 articles published in JoVE Medicine Robotic D3 Partial Duodenal Resection with Primary Side-to-Side Anastomosis Roberto Maria Montorsi1,2,3, Sofia Xenaki1,2,4, Sebastiaan Festen5, Paul Fockens2,6, Barbara A. J. Bastiaansen2,6, Freek Daams2,7, Olivier R. Busch1,2, M. G. Besselink1,2, HPB-Amsterdam, 1Amsterdam UMC, Department of Surgery, University of Amsterdam, 2Cancer Center Amsterdam, 3Department of General and Pancreatic Surgery, The Pancreas Institute, University of Verona Hospital Trust, 4Department of General Surgery, University Hospital of Heraklion Crete, 5Department of Surgery, OLVG, 6Amsterdam UMC, Department of Gastroenterology, University of Amsterdam, 7Amsterdam UMC, Department of Surgery, Vrije Universiteit This protocol presents a case of a robotic partial duodenal resection with primary side-to-side duodeno-jejunal reconstruction in a patient with a 5 cm duodenal stenosis. This is done at the third duodenal segment (D3) after an endoscopic mucosal resection (EMR) for a duodenal polyp. Bioengineering Quantification of Mouse Heart Left Ventricular Function, Myocardial Strain, and Hemodynamic Forces by Cardiovascular Magnetic Resonance Imaging Mariah R. R. Daal1, Gustav J. Strijkers1,2, Claudia Calcagno2, Ruslan R. Garipov3, Rob C. I. Wüst1,4, David Hautemann5, Bram F. Coolen1 1Department of Biomedical Engineering & Physics, Amsterdam University Medical Centers, Amsterdam Cardiovascular Sciences, University of Amsterdam, 2BioMedical Engineering and Imaging Institute, Icahn School of Medicine at Mount Sinai, 3MR Solutions Ltd., 4Laboratory for Myology, Department of Human Movement Sciences, Faculty of Behavioral and Movement Sciences, Vrije Universiteit Amsterdam, 5Medis medical imaging systems B.V. This study describes a comprehensive cardiovascular magnetic resonance imaging (CMR) protocol to quantify the left ventricular functional parameters of the mouse heart. The protocol describes the acquisition, post-processing, and analysis of the CMR images as well as assessment of different cardiac functional parameters. Medicine Laparoscopic Radical Left Pancreatectomy for Pancreatic Cancer: Surgical Strategy and Technique Video Frederique L. Vissers1, Maurice J.W. Zwart1, Alberto Balduzzi1,3, Maarten Korrel1, Sanne Lof2, Mohammad Abu Hilal*2, Marc G. Besselink*1 1Department of Surgery, Cancer Center Amsterdam, Amsterdam UMC, University of Amsterdam, 2Department of Surgery, Southampton University Hospital NHS Foundation Trust, 3General and Pancreatic Surgery Department, Pancreas Institute, University and Hospital Trust of Verona Oncologically safe left pancreatectomy requires radical resection (R0), Gerota’s (perirenal) fascia resection, and adequate lymph node dissection. This study describes the technical details of laparoscopic radical left pancreatectomy (LRLP), used in the first international multicenter randomized trial comparing minimally invasive with open left pancreatectomy for pancreatic cancer, the DIPLOMA trial. Medicine DIPLOMA Approach for Standardized Pathology Assessment of Distal Pancreatectomy Specimens Sanne Lof1, Rushda Rajak2, Frederique L. I. M. Vissers3, Maarten Korrel3, Adrian Bateman2, Johanna Verheij4, Caroline Verbeke5, Ivana Cataldo6, Marc G. Besselink3, Mohammed Abu Hilal1 1Department of Surgery, Southampton University Hospital NHS Foundation Trust, 2Department of Pathology, Southampton University Hospital NHS Foundation Trust, 3Department of Surgery, Cancer Center Amsterdam, Amsterdam UMC, University of Amsterdam, 4Department of Pathology, Cancer Center Amsterdam, Amsterdam UMC, University of Amsterdam, 5Department of Pathology, Oslo University Hospital & University of Oslo, 6Department of Pathology, Ca Foncello Hospital The current study highlights a standardized approach to the macroscopic assessment of distal pancreatectomy specimens for pancreatic ductal adenocarcinoma, with special emphasis on the measurement of pancreatic dimensions and those of other organs, inking of margins, measurement of tumor size and proximity to margins, lymph node sampling and block selection. Medicine Robotic Enucleation of an Intra-Pancreatic Insulinoma in the Pancreatic Head E. Kaçmaz1, M.J.W. Zwart1, A.F. Engelsman1,2, O.R. Busch1, E.J.M. Nieveen van Dijkum1,2, M.G. Besselink1 1Department of Surgery, Cancer Center Amsterdam, Amsterdam UMC, University of Amsterdam, 2ENETS Center of Excellence, Amsterdam UMC, University of Amsterdam Here, we present a robotic approach to enucleate an insulinoma in the pancreatic head. Medicine Robotic Lateral Pancreaticojejunostomy for Chronic Pancreatitis Alberto Balduzzi*1,2, Maurice J. W. Zwart*1, Rens M. A. Kempeneers1, Marja A. Boermeester1, Olivier R. Busch1, Marc G. Besselink1 1Department of Surgery, Amsterdam Gastroenterology and Metabolism, Amsterdam UMC, University of Amsterdam, 2General and Pancreatic Surgery Department, Pancreas Institute, University and Hospital Trust of Verona Robotic lateral pancreaticojejunostomy (RLPJ) may be used in patients with painful, morphine dependent, chronic pancreatitis and a dilated main pancreatic duct. We describe a standardized and reproducible technique for RLPJ, which includes transection of the gastroduodenal artery. Developmental Biology Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids Tânia Martins Garcia1, Marit Navis1, Manon E. Wildenberg1, Ruurd M. van Elburg2, Vanesa Muncan1 1Department of Gastroenterology and Hepatology, Tytgat Institute for Intestinal and Liver Research, Amsterdam UMC, AG&M, University of Amsterdam, 2Department of Pediatrics, Amsterdam UMC, University of Amsterdam This protocol describes how to mimic suckling-to-weaning transition in vitro using mouse late fetal intestinal organoids cultured for 30 days. Bioengineering Bulk Droplet Vitrification for Primary Hepatocyte Preservation Reinier J. de Vries1,2,3, Peony D. Banik1,2, Sonal Nagpal1,2, Lindong Weng1,2, Sinan Ozer1,2, Thomas M. van Gulik3, Mehmet Toner1,2, Shannon N. Tessier1,2, Korkut Uygun1,2 1Center for Engineering in Medicine, Harvard Medical School, Massachusetts General Hospital, 2Shriners Hospitals for Children, Boston, 3Department of Surgery, University of Amsterdam This manuscript describes an ice-free cryopreservation method for large quantities of rat hepatocytes whereby primary cells are pre-incubated with cryoprotective agents at a low concentration and vitrified in large droplets. Bioengineering Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores Juan Wen1, Raymond Pasman1, Erik M.M. Manders*2,3, Peter Setlow*4, Stanley Brul*1 1Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam, 2Van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, 3Confocal.nl BV., 4Dept. of Molecular Biology and Biophysics, UConn Health Germinant receptor proteins cluster in ‘germinosomes’ in the inner membrane of Bacillus subtilis spores. We describe a protocol using super resolution microscopy and fluorescent reporter proteins to visualize germinosomes. The protocol also identifies spore inner membrane domains that are preferentially stained with the membrane dye FM4-64. Bioengineering A Zebrafish Embryo Model for In Vivo Visualization and Intravital Analysis of Biomaterial-associated Staphylococcus aureus Infection Xiaolin Zhang1,2, Leonie de Boer1, Oliver W. Stockhammer1, Dirk W. Grijpma2,3, Herman P. Spaink4, Sebastian A.J. Zaat1 1Department of Medical Microbiology, Amsterdam UMC, 2Technical Medical Center, Department of Biomaterials Science and Technology, University of Twente, 3Department of Biomedical Engineering, W.J. Kolff Institute, University Medical Center Groningen, University of Groningen, 4Institute of Biology, Leiden University The present study describes a zebrafish embryo model for in vivo visualization and intravital analysis of biomaterial-associated infection over time based on fluorescence microscopy. This model is a promising system complementing mammalian animal models such as mouse models for studying biomaterial-associated infections in vivo. Immunology and Infection Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues Remco J. Molenaar1, Mohammed Khurshed1, Vashendriya V.V. Hira1, Cornelis J.F. Van Noorden1 1Cancer Center Amsterdam, Department of Medical Biology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands Here, we present a protocol that can be used to microscopically visualize and quantify activity of dehydrogenases in cells and tissues and its kinetics, function and subcellular localization. Genetics Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector Mariliis Tark-Dame1, Blaise Weber1, Mara de Sain1, Damar Tri Anggoro1, Rechien Bader1, Aimee Walmsley1, Rurika Oka1, Maike Stam1 1Swammerdam Institute for Life Sciences, University of Amsterdam Using a pBIBAC-GW binary vector makes generating transgenic plants with intact single-copy insertions, an easy process. Here, a series of protocols is presented that guide the reader through the process of generating transgenic Arabidopsis plants, and testing the plants for intactness and copy number of the inserts. Chemistry Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns Andrew Jones1, Sercan Pravadali-Cekic1, Stanley Hua1, Danijela Kocic1, Michelle Camenzuli2, Gary Dennis1, Andrew Shalliker1 1School of Science and Health, University of Western Sydney, 2Van′t Hoff Institute for Molecular Sciences, University of Amsterdam A protocol for the use of reaction flow high performance liquid chromatography columns for methods employing post column derivatization (PCD) is presented. Behavior Disrupting Reconsolidation of Fear Memory in Humans by a Noradrenergic β-Blocker Merel Kindt1, Marieke Soeter1, Dieuwke Sevenster1 1Department of Clinical Psychology, University of Amsterdam Disrupting reconsolidation is a promising approach to dampen the behavioral expression of fear memory in patients with anxiety disorders or posttraumatic stress disorder. In a series of human fear conditioning studies we showed that disrupting reconsolidation by the noradrenergic β-blocker propranolol is very effective in erasing conditioned fear responding. Immunology and Infection Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow Jeffrey Kroon*1, Anna E. Daniel*1, Mark Hoogenboezem1, Jaap D. van Buul1 1Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, AMC at University of Amsterdam Leukocytes cross the endothelial monolayer using the paracellular or the transcellular route. We developed a simple assay to follow the distribution of endogenous junctional VE-cadherin and PECAM-1 during leukocyte transendothelial migration under physiological flow to discriminate between the two transmigration routes. Environment Application of Two-spotted Spider Mite Tetranychus urticae for Plant-pest Interaction Studies Marc Cazaux*1,2, Marie Navarro*1,2, Kristie A. Bruinsma*1, Vladimir Zhurov*1, Tara Negrave1, Thomas Van Leeuwen3,4, Vojislava Grbic1,2, Miodrag Grbic1,2 1Department of Biology, The University of Western Ontario, 2Instituto de Ciencias de la Vid y el Vino, 3Department of Crop Protection, Ghent University, 4Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam Protocols for efficient preparation of homogenous samples of spider mites, infestation of experimental plants, and assessment of plant damage, as required for studies of plant-pest interaction were developed. Neuroscience Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy Marijn Schouten*1, Giulia M. R. De Luca*2, Diana K. Alatriste González1, Babette E. de Jong2, Wendy Timmermans1, Hui Xiong1, Harm Krugers1, Erik M. M. Manders2, Carlos P. Fitzsimons1 1Center for Neuroscience, Swammerdam Institute for Life Sciences, University of Amsterdam, 2Van Leeuwenhoek Centre for Advanced Microscopy, Section Molecular Cytology, Swammerdam Institute for Life Sciences, University of Amsterdam This article describes a working protocol to image dendritic spines from hippocampal neurons in vitro using Structured Illumination Microscopy (SIM). Super-resolution microscopy using SIM provides image resolution significantly beyond the light diffraction limit in all three spatial dimensions, allowing the imaging of individual dendritic spines with improved detail. Medicine An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings Justen Manasa1, Siva Danaviah1, Sureshnee Pillay1, Prevashinee Padayachee1, Hloniphile Mthiyane1, Charity Mkhize1, Richard John Lessells1, Christopher Seebregts2, Tobias F. Rinke de Wit3, Johannes Viljoen1, David Katzenstein4, Tulio De Oliveira1 1Africa Centre for Health and Population Studies, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa, 2Unit D11, Jembi Health Systems, 3Academic Medical Center, Department of Global Health, Amsterdam Institute for Global Health and Development (AIGHD), University of Amsterdam, 4Division of Infectious Diseases and Geographic Medicine, Centre for AIDS Research, Stanford Medical School Drug resistance testing for HIV-1 infected individuals failing antiretroviral therapy (ART) can guide future therapies and improve treatment outcomes. Optimizing individual and population health outcomes in high HIV prevalence but resource-limited settings will ultimately require affordable and accessible drug resistance genotyping and interpretation methods. Behavior Training Synesthetic Letter-color Associations by Reading in Color Olympia Colizoli1, Jaap M. J. Murre1, Romke Rouw1 1Brain and Cognition, University of Amsterdam Reading in color is a new method for training letter-color associations that are typically found only in grapheme-color synesthetes. It involves an implicit form of training that has potential for long-term associative training methods because the training is a byproduct of reading and any text can be colored. Immunology and Infection Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells Elisabeth M. Meulenbroek1, Diana Wouters1, Sacha Zeerleder1,2 1Department of Immunopathology, Sanquin Research and Landsteiner Laboratory Academic Medical Center, University of Amsterdam, 2Department of Hematology, Academic Medical Center, University of Amsterdam Here we describe two assays for measuring complement activation induced by antibodies against red blood cells. The major advantage over the current assays is their quantitative and easy-to-interpret nature.