Karmanos Cancer Institute 1 article published in JoVE Cancer Research An Ex Vivo Model of Ovarian Cancer Peritoneal Metastasis Using Human Omentum Terrence Wong1, Roslyn Tedja2, Hussein Chehade2, Robert Morris1, Ayesha B. Alvero2, Gil Mor2 1Department of Gynecologic Oncology, Karmanos Cancer Institute, 2C.S. Mott Center for Human Growth and Development, Department of Obstetrics and Gynecology, Wayne State University School of Medicine This protocol describes the establishment of a three-dimensional (3D) ex vivo model of cancer cell-omentum interaction. The model provides a platform for elucidating pro-tumor mechanisms within the adipose niche and for testing novel therapies.