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Ex vivo Levende Imaging af lungemetastase og deres mikromiljø
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Ex vivo Levende Imaging af lungemetastase og deres mikromiljø

Article DOI: 10.3791/53741-v • 11:19 min • February 3rd, 2016
February 3rd, 2016 •
*1, *1, 1,2, 1,3
1Department of Anatomy, University of California, 2Hubrecht Institute-Royal Dutch Academy of Science and University Medical Center Utrecht, 3Department of Cell and Developmental Biology, Sackler Faculty of Medicine
* These authors contributed equally

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Vi beskriver en forholdsvis enkel fremgangsmåde til ex vivo billeddannelse af tumor celle-stroma interaktioner inden lunge metastase, udnytte fluorescerende reportere i mus. Brug af spinning-disk konfokal mikroskopi, denne teknik muliggør visualisering af levende celler i mindst 4 timer, og kan tilpasses til at studere andre inflammatoriske lungesygdomme.

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Medicin Cancer, spinning disk konfokal mikroskopi metastase immunceller mikromiljø gensplejsede musemodeller.
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