Growth Factor-Coated Bead Placement on Dorsal Forebrain Explants

Published: January 30, 2007

doi:

D. Spencer Currle, Aaron Kolski-Andreaco, Edwin S. Monuki

¹Department of Developmental and Cell Biology,University of California, Irvine (UCI), ²Department of Physiology and Biophysics,University of California, Irvine (UCI), ³Department of Pathology,University of California, Irvine (UCI)

Summary

This video demonstrates two methods for preparing and placing beads, which have been coated with growth factor, on explants of the developing cerebral cortex. These beads can be used to induce spatially restricted gene expression on developing neural tissue such as forebrain explants. Methods are given for using both Affi-Gel beads and heparin acryllic beads.

Disclosures

The authors have nothing to disclose.

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Currle, D. S., Kolski-Andreaco, A., Monuki, E. S. Growth Factor-Coated Bead Placement on Dorsal Forebrain Explants. J. Vis. Exp. (2), e134, doi:10.3791/134 (2007).