Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution (Video) | JoVE

Published: May 05, 2023

doi:

10.3791/200043

Lukasz S. Borowski², Karolina Kasztelan³, Jolanta Czerwinska-Kostrzewska², Roman J. Szczesny

¹Faculty of Biology, Institute of Genetics and Biotechnology,University of Warsaw, ²Institute of Biochemistry and Biophysics,Polish Academy of Sciences, ³International Institute of Molecular and Cell Biology in Warsaw

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Keywords: Primary Antibody Secondary Antibody Transfected HeLa Cells Mitochondrial DNA Distribution BSA PBS Alexa Fluor 488 Alexa Fluor 555 Anti-DNA Antibodies Mitochondrial Transcription Factor A (TFAM)Fluorescence Quantification

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Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution. J. Vis. Exp. (Pending Publication), e200043, doi:10.3791/200043 (2023).

Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution (Video) | JoVE

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