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Biology
Sample Preparation, RNA Extraction, PCR Clean Up, and Quantification for Whole Genome Sequencing of Rabies Virus (RABV) (Video) | JoVE
Published: August 18, 2023
doi:
10.3791/200317
Criselda Bautista
2
,
Gurdeep Jaswant
3,4,5
,
Hollie French
6
,
Kathryn Campbell
,
Rowan Durrant
,
Robert Gifford
6
,
Grace S. N. Kia
8
,
Brian Ogoti
9
,
Katie Hampson
,
Kirstyn Brunker
6
1
School of Biodiversity, One Health & Veterinary Medicine
,
University of Glasgow
,
2
Research Institute for Tropical Medicine
,
3
University of Nairobi, Institute of Tropical and Infectious Diseases
,
4
Tanzania Industrial Research Development Organization
,
5
Ifakara Health Institute
,
6
MRC-University of Glasgow Centre for Virus Research
,
7
Department of Veterinary Public health
,
Ahmadu Bello University
,
8
African Centre of Excellence for Neglected Tropical Diseases and Forensic Biotechnology
,
Ahmadu Bello University
,
9
University of Nairobi, Kenya and Center for Epidemiological Modelling and Analysis
,
University of Nairobi
Tags
Sample Preparation
RNA Extraction
PCR Clean-up
Quantification
Whole Genome Sequencing
Rabies Virus (RABV)
Ceramic Beads
Lysis Buffer
Brain Sample
CDNA Preparation
PCR Amplification
Primer Pools
SPRI Beads
Magnetic Rack
Ethanol Wash
DNA Product
check_url/200317?article_type=t
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Sample Preparation, RNA Extraction, PCR Clean Up, and Quantification for Whole Genome Sequencing of Rabies Virus (RABV).
J. Vis. Exp.
(Pending Publication), e200317, doi:10.3791/200317 (2023).
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