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Immunology and Infection

Monocyte Isolation and Monocyte Differentiation into Monocyte-Derived Dendritic Cells

Published: October 20, 2023

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Vanessa C. C. Luz², Zélia Silva², Patrícia Sobral^2,3, Ankit Tanwar⁵, Rachel L. Paterson, Paula A. Videira^2,7

¹Associate Laboratory i4HB – Institute for Health and Bioeconomy, NOVA School of Science and Technology,Universidade NOVA de Lisboa, ²UCIBIO – Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology,Universidade NOVA de Lisboa, ³LAQV and REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia,Universidade NOVA de Lisboa, ⁴Department of Microbiology and Immunology,Albert Einstein College of Medicine, ⁵Department of Oncology,Albert Einstein College of Medicine, ⁶Stemmatters, Biotecnologia e Medicina Regenerativa SA, Parque de Ciência e Tecnologia Avepark,Zona Industrial da Gandra, ⁷CDG & Allies – Professionals and Patient Associations International Network (CDG & Allies – PPAIN), Department of Life Sciences, NOVA School of Science and Technology,Universidade NOVA de Lisboa

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Keywords: Monocyte Isolation Peripheral Blood Mononuclear Cells Density Gradient Separation CD14 Positive Isolation Magnetic Activated Cell Sorting Monocyte-derived Dendritic Cells

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Monocyte Isolation and Monocyte Differentiation into Monocyte-Derived Dendritic Cells. J. Vis. Exp. (Pending Publication), e200350, doi:10.3791/200350 (2023).

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