Establishing Blood Brain Barrier Monoculture in &#181;SiM Device for Immunocytochemistry Staining and Confocal Analysis

doi:10.3791/200520

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Bioengineering

Establishing Blood Brain Barrier Monoculture in ÂµSiM Device for Immunocytochemistry Staining and Confocal Analysis (Video) | JoVE

Published: January 12, 2024

doi:

10.3791/200520

Molly C. McCloskey, Pelin Kasap, Michelle Trempel, Louis P. Widom, Julia Kuebel, Kaihua Chen, Thomas R. Gaborski, Britta Engelhardt, James L. McGrath

¹Department of Biomedical Engineering,University of Rochester, ²Theodor Kocher Institute,University of Bern, ³Department of Biomedical Engineering,Rochester Institute of Technology

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Blood-brain Barrier Monoculture SiM Device Immunocytochemistry Confocal Analysis Cell Culture Collagen Fibronectin HECSR EECM-BMEC Cell Detachment Endothelial Medium Methanol Fixation PBS Washing Blocking Primary Antibody Secondary Antibody Hoechst Staining

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Establishing Blood Brain Barrier Monoculture in µSiM Device for Immunocytochemistry Staining and Confocal Analysis. J. Vis. Exp. (Pending Publication), e200520, doi:10.3791/200520 (2024).

Establishing Blood Brain Barrier Monoculture in ÂµSiM Device for Immunocytochemistry Staining and Confocal Analysis (Video) | JoVE

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