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Bioengineering
Establishing Blood Brain Barrier Monoculture in µSiM Device for Immunocytochemistry Staining and Confocal Analysis (Video) | JoVE
Published: January 12, 2024
doi:
10.3791/200520
Molly C. McCloskey
,
Pelin Kasap
,
Michelle Trempel
,
Louis P. Widom
,
Julia Kuebel
,
Kaihua Chen
,
Thomas R. Gaborski
,
Britta Engelhardt
,
James L. McGrath
1
Department of Biomedical Engineering
,
University of Rochester
,
2
Theodor Kocher Institute
,
University of Bern
,
3
Department of Biomedical Engineering
,
Rochester Institute of Technology
Tags
Blood-brain Barrier
Monoculture
SiM Device
Immunocytochemistry
Confocal Analysis
Cell Culture
Collagen
Fibronectin
HECSR
EECM-BMEC
Cell Detachment
Endothelial Medium
Methanol Fixation
PBS Washing
Blocking
Primary Antibody
Secondary Antibody
Hoechst Staining
check_url/200520?article_type=t
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Establishing Blood Brain Barrier Monoculture in µSiM Device for Immunocytochemistry Staining and Confocal Analysis.
J. Vis. Exp.
(Pending Publication), e200520, doi:10.3791/200520 (2024).
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