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Biology
Single-Cell Dissociation of Murine Islets for Flow Cytometric Analysis of β-Cell Mitophagy (Video) | JoVE
Published: September 15, 2023
doi:
10.3791/200660
Elena Levi-D’Ancona
2
,
Vaibhav Sidarala
,
Scott A. Soleimanpour
3,4
1
Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes
,
University of Michigan, Ann Arbor
,
2
Graduate Program in Immunology
,
University of Michigan Medical School
,
3
Department of Molecular and Integrative Physiology
,
University of Michigan, Ann Arbor
,
4
VA Ann Arbor Healthcare System
Tags
Keywords: Single-cell Dissociation
Murine Islets
Flow Cytometric Analysis
β-cell Mitophagy
Islet Culture
High-fat Diet
Valinomycin
Trypsin
Cell Staining
Flow Cytometry
check_url/200660?article_type=t
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Single-Cell Dissociation of Murine Islets for Flow Cytometric Analysis of β-Cell Mitophagy.
J. Vis. Exp.
(Pending Publication), e200660, doi:10.3791/200660 (2023).
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