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Immunology and Infection
Mouse Splenic Single-Cell Suspension Generation and FACS Staining for Flow Cytometry-Based Sorting (Video) | JoVE
Published: February 23, 2024
doi:
10.3791/200687
Katharina M. Glaser*
1
,
Mirijam Egg*
1
,
Sebastian Hobitz
,
Michael Mitterer
,
Dominik Schain-Zota
,
Katharina Schönberger
,
Konrad Schuldes
,
Nina Cabezas-Wallscheid
,
Tim Lämmermann
,
Angelika Rambold
,
Joerg M. Buescher
1
Max Planck Institute of Immunobiology and Epigenetics
Tags
Mouse
Spleen
Single-cell Suspension
FACS
Flow Cytometry
Cell Strainer
Centrifugation
Lysis
Antibody Staining
FACS Tube
Filtration
check_url/200687?article_type=t
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Mouse Splenic Single-Cell Suspension Generation and FACS Staining for Flow Cytometry-Based Sorting.
J. Vis. Exp.
(Pending Publication), e200687, doi:10.3791/200687 (2024).
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