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Biochemistry
Substrate-Uncoupler-Inhibitor-Titration for Mitochondrial Respiration Analysis in Drosophila melanogaster (Video) | JoVE
Published: November 10, 2023
doi:
10.3791/200718
Paula Michelotti*
1,2
,
Tâmie Duarte*
1,2
,
Cristiane L. Dalla Corte
2
1
Laboratory of Experimental Biochemistry and Toxicology, Department of Biochemistry and Molecular Biology, Center of Natural and Exact Sciences
,
Federal University of Santa Maria
,
2
Graduate Program in Biological Sciences: Toxicological Biochemistry
,
Federal University of Santa Maria
Tags
Keywords: Mitochondrial Respiration
Drosophila Melanogaster
Substrate
Uncoupler
Inhibitor
Titration
Polarographic Oxygen Sensors
MiR05 Buffer
High-resolution Respirometry
Digitonin
Pyruvate
Malate
Proline
ADP
Succinate
Oligomycin
FCCP
Rotenone
Malonate
Antimycin
OXPHOS CI
OXPHOS CII
ETS CI
ETS CII
ATP Synthesis
OXPHOS/LEAK Ratio
check_url/200718?article_type=t
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Substrate-Uncoupler-Inhibitor-Titration for Mitochondrial Respiration Analysis in
Drosophila melanogaster
.
J. Vis. Exp.
(Pending Publication), e200718, doi:10.3791/200718 (2023).
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