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Biochemistry
Crystallization of Heterodimeric ABCG5/G8 Protein in Lipidic Bicelle Using Hanging Drop Vapor Diffusion (Video) | JoVE
Published: August 25, 2023
doi:
10.3791/200737
Sarah Wazir
,
Danny Farhat
,
Mahalashmi Srinivasan
2
,
Jyh-Yeuan Lee
1
Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine
,
University of Ottawa
,
2
Department of Biochemistry, Faculty of Medicine and Dentistry
,
University of Alberta
Tags
Keywords: Crystallization
Heterodimeric
ABCG5/G8 Protein
Lipidic Bicelle
Hanging Drop Vapor Diffusion
Bicelle Stock Solution
DMPC
CHAPSO
Protein-bicelle Mixture
Crystallization Reservoir Solution
Crystal Growth
Sodium Malate
Cryo Loops
Data Collection
check_url/200737?article_type=t
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Crystallization of Heterodimeric ABCG5/G8 Protein in Lipidic Bicelle Using Hanging Drop Vapor Diffusion.
J. Vis. Exp.
(Pending Publication), e200737, doi:10.3791/200737 (2023).
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