One and Two-Step Method of Rat Neutrophil Isolation for Harvesting Neutrophil Extracellular Traps (NETs) - JoVE

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One and Two-Step Method of Rat Neutrophil Isolation for Harvesting Neutrophil Extracellular Traps (NETs)

Published: April 26, 2024

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Xiangfeng Gong*¹, Yutao Sun*¹, Xiaoxin Zhang³, Zhenghua Xiao⁴, Li He⁴, Chaoyi Qin⁴

¹Department of Cardiovascular Surgery, West China Hospital,Sichuan University, ²Department of Integrated Traditional Chinese and Western Medicine, Sichuan Provincial Pancreatitis Centre, West China Hospital,Sichuan University, ³West China-Liverpool Biomedical Research Centre, West China Hospital,Sichuan University, ⁴Cardiovascular Surgery Research Laboratory, West China Hospital,Sichuan University

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Keywords: Rat Neutrophil Isolation Neutrophil Extracellular Traps (NETs)Bone Marrow Harvest Density Gradient Centrifugation One-step Method Two-step Method RPMI Media Red Blood Cell Lysis Buffer Percoll Reagent HBSS Cell Viability Hemocytometer Trypan Blue Staining

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One and Two-Step Method of Rat Neutrophil Isolation for Harvesting Neutrophil Extracellular Traps (NETs). J. Vis. Exp. (Pending Publication), e200810, doi:10.3791/200810 (2024).

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