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Immunology and Infection
Generation of 2D Monolayers from 3D Bovine Ileal Enteroids (Video) | JoVE
Published: April 05, 2024
doi:
10.3791/200825
Deborah Molehin
,
Jack Guinan
,
Brina Lopez
1
Department of Farm Animal Medicine, College of Veterinary Medicine
,
Midwestern University
Tags
Keywords: 2D Monolayers
3D Bovine Ileal Enteroids
Basement Membrane Extracellular Matrix
BME
Cell Culture
Cell Dissociation
Cell Suspension
Enteroid Fragments
Enteroid Growth Media
FBS Wash Media
Organoid Growth Media
TrypLE Express Enzyme
Trypan Blue Dye Exclusion
check_url/200825?article_type=t
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Generation of 2D Monolayers from 3D Bovine Ileal Enteroids.
J. Vis. Exp.
(Pending Publication), e200825, doi:10.3791/200825 (2024).
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