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Collection of Primordial Germ Cells from Drosophila Donor Embryos (Video) | JoVE

Published: December 01, 2023

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Kaori Nishimura*¹, Miho Asaoka*², Yurina Sakamaki*², Tatsuya Fukumoto*^3,4, Daisuke Tanaka, Satoru Kobayashi, Toshiyuki Takano-Shimizu-Kouno

¹KYOTO Drosophila Stock Center,Kyoto Institute of Technology, ²Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),University of Tsukuba, ³Research Center of Genetic Resources,National Agriculture and Food Research Organization, ⁴Shizuoka Prefectural Ogasa High School

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Keywords: Primordial Germ Cells PGCs Drosophila Embryos Micro Manipulator Forceps PGC Collection Glass Slide Transplantation Needle Somatic Cell Layer Silicone Oil Yolk Contaminants

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Collection of Primordial Germ Cells from Drosophila Donor Embryos. J. Vis. Exp. (Pending Publication), e200839, doi:10.3791/200839 (2023).

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