Flow Cytometry and Data Analysis of Murine Brain Histone Post-Translational Modifications

doi:10.3791/200982

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Neuroscience

Flow Cytometry and Data Analysis of Murine Brain Histone Post-Translational Modifications

Published: September 15, 2023

doi:

10.3791/200982

Morgan Towriss, Jennifer Kim, Annie Vogel Ciernia

¹Department of Biochemistry and Molecular Biology, Djavad Mowafaghian Centre for Brain Health,University of British Columbia, ²Graduate Program in Neuroscience, Djavad Mowafaghian Centre for Brain Health,University of British Columbia

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Flow Cytometry Murine Brain Histone Post-translational Modifications Antibody Panel Compensation Matrix Rainbow Fluorescent Beads Photomultiplier Tube Voltage Compensation Beads Side Scatter Forward Scatter Singlet Cells FMO Microglia P2RY12 Protein Expression Median Fluorescence Intensity (MFI)Lipopolysaccharide Histone 3 Lysine 27 Acetylation Histogram Analysis

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Flow Cytometry and Data Analysis of Murine Brain Histone Post-Translational Modifications. J. Vis. Exp. (Pending Publication), e200982, doi:10.3791/200982 (2023).

Flow Cytometry and Data Analysis of Murine Brain Histone Post-Translational Modifications

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