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Biology
In Vitro Human Pluripotent Stem Cell Differentiation into Insulin-Producing Pancreatic Cells
Published: February 02, 2024
doi:
10.3791/200998
Ines Cherkaoui
,
Qian Du
,
Dieter Egli
,
Shivani Misra
3
,
Guy A. Rutter
4,5
1
Department of Metabolism, Digestion and Reproduction, Faculty of Medicine
,
Cell Biology and Functional Genomics
,
2
Departments of Pediatrics, Naomi Berrie Diabetes Center, Obstetrics and Gynecology, Columbia Stem Cell Initiative, Columbia University Irving Medical Center
,
Columbia University
,
3
Department of Diabetes
,
Imperial College Healthcare NHS Trust
,
4
Faculté de Médicine
,
Université de Montréal
,
5
Lee Kong Chian Imperial Medical School
,
Nanyang Technological University
Tags
In Vitro
Human Pluripotent Stem Cells
Insulin-producing
Pancreatic Cells
Cell Passage
Coating Solution
Dissociation Solution
Trypan Blue
Hemocytometer
Rock Inhibitor
Basal Medium
Supplements
Washing Medium
Day One Medium
Pancreatic Progenitor Medium
Dissociation Buffer
Microwells
Anti-Adherence Rinsing Solution
check_url/200998?article_type=t
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In Vitro Human Pluripotent Stem Cell Differentiation into Insulin-Producing Pancreatic Cells.
J. Vis. Exp.
(Pending Publication), e200998, doi:10.3791/200998 (2024).
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