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Immunology and Infection

Live Imaging of Virus-Infected Human Intestinal Organoid Monolayers (Video) | JoVE

Published: January 19, 2024

doi:

J. Thomas Gebert, Francesca J. Scribano, Kristen A. Engevik, Joseph M. Hyser

¹Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology,Baylor College of Medicine

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Keywords: Live Imaging Virus-infected Human Intestinal Organoid Monolayers Epifluorescence Microscope Stage Top Incubator Bright Field Illumination Fluorescent Calcium Sensor Fluorescently Tagged Viruses Amino Fluorescent Staining Formaldehyde Ammonium Chloride Triton X-100 Blocking Solution Primary Antibodies Secondary Antibodies DAPI Rotavirus Infection HIO Monolayer

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Live Imaging of Virus-Infected Human Intestinal Organoid Monolayers. J. Vis. Exp. (Pending Publication), e201233, doi:10.3791/201233 (2024).

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