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Biology
Monitoring Actin Disassembly with Time-lapse Microscopy
Published: November 08, 2006
doi:
10.3791/66
Hao Yuan Kueh
1
Dept. of Systems Biology
,
Harvard Medical School
Protocol
Construct a flow chamber as shown in the video. Make sure that the parafilm seal is tight and use washed coverslips.
Incubate actin-binding agent in the chamber for 5-10′.
Block non-specific binding sites on the glass coverslip with a blocking protein.
Polymerize actin inside the chamber by flowing in G-actin in polymerizing buffer.
Washout unpolymerized actin by flowing in excess buffer.
Tags
Monitoring
Actin Disassembly
Time-lapse Microscopy
check_url/66?article_type=t
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Cite This Article
Kueh, H. Y. Monitoring Actin Disassembly with Time-lapse Microscopy.
J. Vis. Exp.
(1), e66, doi:10.3791/66 (2006).
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