Jan Polák Department of Pathophysiology, Third Faculty of Medicine Charles University Biography Publications Institution JoVE Articles Jan Polák has not added a biography. If you are Jan Polák and would like to personalize this page please email our Author Liaison for assistance. Publications Obstructive Sleep Apnoea Increases Lipolysis and Deteriorates Glucose Homeostasis in Patients with Type 2 Diabetes Mellitus Scientific Reports. Feb, 2021 | Pubmed ID: 33574418 Technical Feasibility and Physiological Relevance of Hypoxic Cell Culture Models Frontiers in Endocrinology. 2020 | Pubmed ID: 32153502 Intermittent Hypoxia Stimulates Lipolysis, But Inhibits Differentiation and Lipogenesis in 3T3-L1 Cells Metabolic Syndrome and Related Disorders. 04, 2020 | Pubmed ID: 31928504 Hypoxia Modulates Effects of Fatty Acids on NES2Y Human Pancreatic β-cells International Journal of Molecular Sciences. Jul, 2019 | Pubmed ID: 31336948 The Clinical Impact of Systematic Screening for Obstructive Sleep Apnea in a Type 2 Diabetes Population-Adherence to the Screening-Diagnostic Process and the Acceptance and Adherence to the CPAP Therapy Compared to Regular Sleep Clinic Patients Frontiers in Endocrinology. 2018 | Pubmed ID: 30555416 The Effect of Hypoxia and Metformin on Fatty Acid Uptake, Storage, and Oxidation in L6 Differentiated Myotubes Frontiers in Endocrinology. 2018 | Pubmed ID: 30386299 Blockade of Endothelin-1 Receptor Type B Ameliorates Glucose Intolerance and Insulin Resistance in a Mouse Model of Obstructive Sleep Apnea Frontiers in Endocrinology. 2018 | Pubmed ID: 29896159 Commentary: Baseline Adiponectin Concentration and Clinical Outcomes Among Patients with Diabetes and Recent Acute Coronary Syndrome in the EXAMINE Trial Frontiers in Endocrinology. 2018 | Pubmed ID: 29666606 An Innovative Intermittent Hypoxia Model for Cell Cultures Allowing Fast Po Oscillations with Minimal Gas Consumption American Journal of Physiology. Cell Physiology. Oct, 2017 | Pubmed ID: 28747336 Increased Incretin But Not Insulin Response After Oral Versus Intravenous Branched Chain Amino Acids Annals of Nutrition & Metabolism. 2017 | Pubmed ID: 28595189 Electrophoretic Stacking for Sensitive Determination of Antibiotic Ceftazidime in Human Blood and Microdialysates from Diabetic Foot Analytica Chimica Acta. Oct, 2016 | Pubmed ID: 27720117 Screening for Obstructive Sleep Apnea in Type 2 Diabetes Patients - Questionnaires Are Not Good Enough Frontiers in Endocrinology. 2016 | Pubmed ID: 27679608 Screening for Obstructive Sleep Apnea Syndrome in Patients with Type 2 Diabetes Mellitus: a Prospective Study on Sensitivity of Berlin and STOP-Bang Questionnaires Sleep Medicine. 10, 2016 | Pubmed ID: 27613528 The Effect of Hypoxia and Re-oxygenation on Adipose Tissue Lipolysis in COPD Patients The European Respiratory Journal. 10, 2016 | Pubmed ID: 27587548 Mitochondrial Probe Methyltriphenylphosphonium (TPMP) Inhibits the Krebs Cycle Enzyme 2-Oxoglutarate Dehydrogenase PloS One. 2016 | Pubmed ID: 27537184 Adipogenesis, Lipogenesis and Lipolysis is Stimulated by Mild but Not Severe Hypoxia in 3T3-L1 Cells Biochemical and Biophysical Research Communications. 09, 2016 | Pubmed ID: 27498031 The Effect of Pericellular Oxygen Levels on Proteomic Profile and Lipogenesis in 3T3-L1 Differentiated Preadipocytes Cultured on Gas-Permeable Cultureware PloS One. 2016 | Pubmed ID: 27023342 Inhibition of Lipolysis Ameliorates Diabetic Phenotype in a Mouse Model of Obstructive Sleep Apnea American Journal of Respiratory Cell and Molecular Biology. 08, 2016 | Pubmed ID: 26978122 Licensing Regulations for Diabetes and Driving--Two Sides of the Same Coin? Diabetes Research and Clinical Practice. Mar, 2016 | Pubmed ID: 26810271 Fear of Driving License Withdrawal in Patients with Insulin-treated Diabetes Mellitus Negatively Influences Their Decision to Report Severe Hypoglycemic Events to Physicians Patient Preference and Adherence. 2015 | Pubmed ID: 26491264 The Impact of Sleep Disorders on Glucose Metabolism: Endocrine and Molecular Mechanisms Diabetology & Metabolic Syndrome. 2015 | Pubmed ID: 25834642 System for Exposing Cultured Cells to Intermittent Hypoxia Utilizing Gas Permeable Cultureware General Physiology and Biophysics. Jul, 2015 | Pubmed ID: 25816360 Effect of Short-term Hyperglycemia on Adipose Tissue Fluxes of Selected Cytokines in Vivo During Multiple Phases of Diet-induced Weight Loss in Obese Women The Journal of Clinical Endocrinology and Metabolism. May, 2015 | Pubmed ID: 25751103 Soluble CD163 is Associated with CD163 MRNA Expression in Adipose Tissue and with Insulin Sensitivity in Steady-state Condition but Not in Response to Calorie Restriction The Journal of Clinical Endocrinology and Metabolism. Mar, 2014 | Pubmed ID: 24423341 Could Continuous Glucose Monitoring Facilitate Identifying Diabetes Patients with a Higher Risk of Hypoglycemia During Driving? Journal of Diabetes Science and Technology. Nov, 2013 | Pubmed ID: 24351191 Intermittent Hypoxia Impairs Glucose Homeostasis in C57BL6/J Mice: Partial Improvement with Cessation of the Exposure Sleep. Oct, 2013 | Pubmed ID: 24082307 The Impact of Full-Length, Trimeric and Globular Adiponectin on Lipolysis in Subcutaneous and Visceral Adipocytes of Obese and Non-Obese Women PloS One. 2013 | Pubmed ID: 23805277 Change in Proportional Protein Intake in a 10-week Energy-restricted Low- or High-fat Diet, in Relation to Changes in Body Size and Metabolic Factors Obesity Facts. 2013 | Pubmed ID: 23711745 TFAP2B Influences the Effect of Dietary Fat on Weight Loss Under Energy Restriction PloS One. 2012 | Pubmed ID: 22952648 Catecholamine and Insulin Control of Lipolysis in Subcutaneous Adipose Tissue During Long-term Diet-induced Weight Loss in Obese Women American Journal of Physiology. Endocrinology and Metabolism. Jan, 2012 | Pubmed ID: 22028414 Post-prandial Endothelial Dysfunction is Ameliorated Following Weight Loss in Obese Premenopausal Women Medical Science Monitor : International Medical Journal of Experimental and Clinical Research. Nov, 2011 | Pubmed ID: 22037742 Lipid Mobilization in Subcutaneous Adipose Tissue During Exercise in Lean and Obese Humans. Roles of Insulin and Natriuretic Peptides American Journal of Physiology. Endocrinology and Metabolism. Aug, 2010 | Pubmed ID: 20484012 Effect of Hyperinsulinemia and Very-low-calorie Diet on Interstitial Cytokine Levels in Subcutaneous Adipose Tissue of Obese Women American Journal of Physiology. Endocrinology and Metabolism. Nov, 2009 | Pubmed ID: 19724021 Macrophages and Adipocytes in Human Obesity: Adipose Tissue Gene Expression and Insulin Sensitivity During Calorie Restriction and Weight Stabilization Diabetes. Jul, 2009 | Pubmed ID: 19401422 Secretion of Adiponectin Multimeric Complexes from Adipose Tissue Explants is Not Modified by Very Low Calorie Diet European Journal of Endocrinology. Apr, 2009 | Pubmed ID: 19155318 Multiple Roles of Leptin in Blood Pressure Regulation, from Central to Peripheral Mechanisms American Journal of Hypertension. Nov, 2008 | Pubmed ID: 18941446 Total Adiponectin and Adiponectin Multimeric Complexes in Relation to Weight Loss-induced Improvements in Insulin Sensitivity in Obese Women: the NUGENOB Study European Journal of Endocrinology. Apr, 2008 | Pubmed ID: 18362301 Acute Exposure to Long-chain Fatty Acids Impairs {alpha}2-adrenergic Receptor-mediated Antilipolysis in Human Adipose Tissue Journal of Lipid Research. Oct, 2007 | Pubmed ID: 17625217 The Atrial Natriuretic Peptide- and Catecholamine-induced Lipolysis and Expression of Related Genes in Adipose Tissue in Hypothyroid and Hyperthyroid Patients American Journal of Physiology. Endocrinology and Metabolism. Jul, 2007 | Pubmed ID: 17389707 An Increase in Plasma Adiponectin Multimeric Complexes Follows Hypocaloric Diet-induced Weight Loss in Obese and Overweight Pre-menopausal Women Clinical Science (London, England : 1979). Jun, 2007 | Pubmed ID: 17201694 Effect of Aerobic Training on Plasma Levels and Subcutaneous Abdominal Adipose Tissue Gene Expression of Adiponectin, Leptin, Interleukin 6, and Tumor Necrosis Factor Alpha in Obese Women Metabolism: Clinical and Experimental. Oct, 2006 | Pubmed ID: 16979409 Genetic Polymorphisms and Weight Loss in Obesity: a Randomised Trial of Hypo-energetic High- Versus Low-fat Diets PLoS Clinical Trials. Jun, 2006 | Pubmed ID: 16871334 Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells Moustafa Elkalaf1,2, Karolína Vaněčková1,2, Pavla Staňková1, Zuzana Červinková1, Jan Polák*2,3, Otto Kučera*1 1Department of Physiology, Faculty of Medicine in Hradec Králové, Charles University, 2Department of Pathophysiology, Third Faculty of Medicine, Charles University, 3Department of Internal Medicine, University Hospital Kralovske Vinohrady JoVE 62902 Biology
Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells Moustafa Elkalaf1,2, Karolína Vaněčková1,2, Pavla Staňková1, Zuzana Červinková1, Jan Polák*2,3, Otto Kučera*1 1Department of Physiology, Faculty of Medicine in Hradec Králové, Charles University, 2Department of Pathophysiology, Third Faculty of Medicine, Charles University, 3Department of Internal Medicine, University Hospital Kralovske Vinohrady JoVE 62902 Biology