A Protein Suspension-Trapping Sample Preparation for Tear Proteomics by Liquid Chromatography-Tandem Mass Spectrometry

Jimmy Sung-Hei Tse; Ying-Hon Sze; Jimmy Ka-Wai Cheung; King-Kit Li; Thomas Chuen Lam

doi:10.3791/64617

Non-Invasive Biomarker Discovery for Tear Proteomics Using LC-MS

December 01, 2023

Advancing Tear Fluid Analysis Using a Standardized Protocol for Proteomics Research (Video) | JoVE

02:15 min

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Collection of Human Tear Fluid Using Schirmer Strip

01:43 min

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Protein Extraction from Human Tear Using Schirmer Strip (Video) | JoVE

01:19 min

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Suspension-Trapping Sample Preparation of Human Tear Protein (Video) | JoVE

03:45 min

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Jimmy Sung-Hei Tse*¹, Ying-Hon Sze*^1,2, Jimmy Ka-Wai Cheung², King-Kit Li, Thomas Chuen Lam^2,3,4

¹Centre for Myopia Research, School of Optometry,The Hong Kong Polytechnic University, ²Centre for Eye and Vision Research (CEVR), ³Research Centre for SHARP Vision (RCSV),The Hong Kong Polytechnic University, ⁴Research Centre for Chinese Medicine Innovation (RCMI),The Hong Kong Polytechnic University

Summary

This protocol describes a method for collecting tear samples using Schirmer strips and an integrated quantitative workflow for discovering non-invasive tear protein biomarkers. The suspension trapping sample preparation workflow enables fast and robust tear sample preparation and mass spectrometric analysis, resulting in higher peptide recovery yields and protein identification than standard in-solution procedures.