<em>Agrobacterium tumefaciens</em>-Mediated Genetic Engineering of Green Microalgae, <em>Chlorella vulgaris</em>

M. Reza Roushan; Chuchi Chen; Parisa Ahmadi; Valerie C. A. Ward

doi:10.3791/65382

Stable Gene Integration in Chlorella vulgaris Using Agrobacterium tumefaciens

October 27, 2023

Optimized Transformation Protocol for Chlorella vulgaris Using Agrobacterium tumefaciens (Video) | JoVE

01:23 min

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Preparation of Electrocompetent Agrobacterium tumefaciens Culture for Microalgal Genetic Engineering (Video) | JoVE

01:40 min

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Electroporation of Agrobacterium tumefaciens for Genetic Engineering of Microalgal Cells (Video) | JoVE

01:16 min

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Agrobacterium-Mediated Transformation of Chlorella vulgaris and Confirmation of Gene Integration via Colony PCR (CPCR) (Video) | JoVE

04:02 min

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M. Reza Roushan, Chuchi Chen, Parisa Ahmadi, Valerie C. A. Ward

¹Department of Chemical Engineering,University of Waterloo

Summary

This protocol outlines the utilization of Agrobacterium tumefaciens-mediated transformation (AMT) for integrating gene(s) of interest into the nuclear genome of the green microalgae Chlorella vulgaris, leading to the production of stable transformants.