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High-Throughput Screening Analysis for 4B Cysteine Peptidase Autophagy Inhibitor
June 30, 2023
基于细胞的自噬相关 4B 半胱氨酸肽酶抑制剂的药物筛选
03:29 min
•
自噬蛋白酶标签化合物添加和细胞上清液收获用于药物筛选
01:48 min
•
荧光素酶检测自噬蛋白酶ATG4B
02:17 min
•
荧光素酶检测自噬蛋白酶ATG4B的图像采集与分析
02:43 min
•
Denise R. B. Pilger
,
Christin Luft
,
Robin Ketteler
2
1
Laboratory for Molecular Cell Biology
,
University College London
,
2
Department of Human Medicine
,
Medical School Berlin
Summary
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在这里,我们描述了在半自动、高通量筛选形式中使用基于荧光素酶的报告基因测定的详细方案。
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