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A Detailed Guide for Preparing Hard Palm Seeds for MALDI-IMS Analysis

June 30, 2023
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Author Spotlight: A Tailor-Made Sample Preparation Approach for Enhanced MALDI-IMS Analysis of Hard Palm Seeds

02:20 min
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Seed Sectioning Protocol and Matrix Deposition Technique for MALDI-IMS Analysis of Hard Palm Seeds

02:23 min
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Image Acquisition and MALDI-IMS Analysis of Hard Palm Seeds

03:16 min
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1Laboratório de Biocatálise (LABIC),Instituto Nacional de Tecnologia, 2Departamento de Bioquímica, Instituto de Química,Universidade Federal do Rio de Janeiro, 3Centro de Espectrometria de Massas de Biomoléculas, Instituto de Biofísica Carlos Chagas Filho,Universidade Federal do Rio de Janeiro

Summary

Automatic Translation

This protocol aimed to describe detailed guidance on the preparation of hard seed sample sections with low water content for MALDI-IMS analysis, maintaining analytes’ original distribution and abundance and providing high-quality signal and spatial resolution.

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