Sample Preparation for <em>In Situ</em> Cryotomography of Mammalian Cells

Noah Weber; Brennan Hinks; Jacob Jensen; Thomas Lidahl; Luiza Mendon&#231;a

doi:10.3791/65697

Preparation of Electron Microscopy Grids for In Situ Cellular Cryotomography

December 15, 2023

Optimizing Grid Preparation for Enhanced Cryoelectron Tomography (Video) | JoVE

01:19 min

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Preparing Electron Microscopy (EM) Grids for Cellular Cryotomography (Video) | JoVE

02:29 min

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Cell Seeding and Transfection on Electron Microscopy (EM) Grids for Cryotomography (Video) | JoVE

02:04 min

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Plunge Freezing of Electron Microscopy (EM) Grids for Cryotomography (Video) | JoVE

02:45 min

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Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen, Thomas Lidahl³, Luiza Mendonça

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

Summary

This method provides an accessible and flexible protocol for the preparation of electron microscopy (EM) grids for in situ cellular cryotomography and correlative light and electron microscopy (CLEM).