Fiber Type Identification of Human Skeletal Muscle

Heidy K. Latchman; Stefan G. Wette; Dion J. Ellul; Robyn M. Murphy; Noni T. Frankenberg

doi:10.3791/65750

MyDoBID- A Novel Technique for Identifying Muscle Fiber Types Efficiently

September 22, 2023

Fiber Type Identification of Human Skeletal Muscle

02:11 min

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Isolation of Single-Fiber from Freeze-Dried Human Skeletal Muscle

01:15 min

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MHCI and MHCII Analysis of Single Fibers Isolated from Freeze-Dried Muscle Samples Using Dot Blot

04:08 min

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Heidy K. Latchman*¹, Stefan G. Wette*¹, Dion J. Ellul, Robyn M. Murphy, Noni T. Frankenberg

¹Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, School of Agriculture, Biomedicine and Environment,La Trobe University

Summary

This protocol demonstrates single-fiber isolation from freeze-dried human skeletal muscle and fiber-type classification according to Myosin heavy chain (MHC) isoform using the dot blotting technique. Identified MHC I and II fiber samples can then be further analyzed for fiber type-specific differences in protein expression using western blotting.

MyDoBID- A Novel Technique for Identifying Muscle Fiber Types Efficiently

Fiber Type Identification of Human Skeletal Muscle

Isolation of Single-Fiber from Freeze-Dried Human Skeletal Muscle

MHCI and MHCII Analysis of Single Fibers Isolated from Freeze-Dried Muscle Samples Using Dot Blot

Summary

Article