Detection of G1 Phase Single-Stranded DNA Foci via RPA2 Immunofluorescent Staining

December 22, 2023

在细胞周期的 G1 期可视化单链 DNA 病灶

G1同步细胞的制备以测量遗传毒性应激下ssDNA病灶的形成

Qingyue Zhang², Marc A. Kerzhnerman², Nelson García-Vázquez^2,3, Gergely Rona^2,4,5

¹Department of Biochemistry and Molecular Pharmacology,NYU Grossman School of Medicine, ²The Laura and Isaac Perlmutter Cancer Center,NYU Langone Health, ³Department of Cell Biology,NYU Grossman School of Medicine, ⁴Howard Hughes Medical Institute,NYU Grossman School of Medicine, ⁵Institute of Enzymology, Centre of Excellence of the Hungarian Academy of Sciences,HUN-REN Research Centre for Natural Sciences

Summary

以下方案介绍了利用细胞周期同步和RPA2免疫荧光染色检测细胞周期G1期的单链DNA病灶。

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