Arteriovenous Metabolomics to Measure <em>In Vivo</em> Metabolite Exchange in Brown Adipose Tissue

Sanghun Lee; Gyumin Lim; Soyeon Kim; Hyoju Kim; Yeon Jin Roh; Wantae Kim; Dong Wook Choi; Su Myung Jung

doi:10.3791/66012

Exploring Metabolite Exchange in Brown Adipose Tissue

October 06, 2023

Arteriovenøs metabolomics til måling af in vivo metabolitudveksling i brunt fedtvæv

00:58 min

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Arteriovenous Blood Sampling Technique in Mice Using Sulzer's Vein and Left Ventricle for Metabolomic Analysis

02:00 min

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Sanghun Lee*¹, Gyumin Lim*^2,3, Soyeon Kim*¹, Hyoju Kim, Yeon Jin Roh, Wantae Kim, Dong Wook Choi, Su Myung Jung

¹Department of Biological Sciences,Sungkyunkwan University (SKKU), ²Department of Biochemistry, College of Natural Sciences,Chungnam National University, ³Department of Biotechnology, College of Life Sciences and Biotechnology,Korea University

Summary

I denne protokol skitseres metoder, der er relevante for BAT-optimeret arteriovenøs metabolomics ved anvendelse af GC-MS i en musemodel. Disse metoder giver mulighed for erhvervelse af værdifuld indsigt i BAT-medieret metabolitudveksling på organismeniveau.

Exploring Metabolite Exchange in Brown Adipose Tissue

Arteriovenøs metabolomics til måling af in vivo metabolitudveksling i brunt fedtvæv

Arteriovenous Blood Sampling Technique in Mice Using Sulzer's Vein and Left Ventricle for Metabolomic Analysis

Summary

Article